Literature DB >> 761403

The analysis of alkaline phosphatase isoenzyme using 4-methylumbelliferyl phosphate as substrate on a cellulose acetate membrane.

F Watanabe, M Takano, F Tanaka, N Amino, C Hayashi, K Miyai.   

Abstract

A new procedure is established for the analysis of alkaline phosphatase isoenzymes. The electrophoretic separation on cellulose acetate membrane coupled with the detection of alkaline phosphatase activity with 4-methyl-umbelliferyl phosphate as a substrate is described. The proposed method would be useful for the analysis of sample of micro-scale quantities and low activities.

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Year:  1979        PMID： 761403     DOI： 10.1016/0009-8981(79)90483-2

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  3 in total

1.  Cell polarization is required for ricin sensitivity in a Caco-2 cell line selected for ricin resistance.

Authors:  M R Jackman; J A Ellis; S R Gray; W Shurety; J P Luzio
Journal:  Biochem J       Date:  1999-07-15       Impact factor: 3.857

2.  The post-synthetic sorting of endogenous membrane proteins examined by the simultaneous purification of apical and basolateral plasma membrane fractions from Caco-2 cells.

Authors:  J A Ellis; M R Jackman; J P Luzio
Journal:  Biochem J       Date:  1992-04-15       Impact factor: 3.857

3.  An impedimetric determination of alkaline phosphatase activity based on the oxidation reaction mediated by Cu2+ bound to poly-thymine DNA.

Authors:  Joon Young Lee; Jun Ki Ahn; Ki Soo Park; Hyun Gyu Park
Journal:  RSC Adv       Date:  2018-03-21       Impact factor: 3.361
  3 in total

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