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Literature DB >> 7612245

Isolation of SagI, a new HaeIII isoschizomer from Streptococcus agalactiae.

Abstract

A new HaeIII isoschizomer from Streptococcus agalactiae was isolated by a single-step purification method. The highly active restriction endonuclease, SagI, was free of nonspecific nuclease activity and was suitable for use in molecular biology procedures. The rapid isolation procedure may be applicable for the recovery of other restriction endonucleases from bacteria.

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Year: 1995 PMID： 7612245 DOI： 10.1007/BF00172825

Source DB: PubMed Journal: Appl Microbiol Biotechnol ISSN： 0175-7598 Impact factor: 4.813

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References

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Isolation of SagI, a new HaeIII isoschizomer from Streptococcus agalactiae.

1. A new sequence-specific endonuclease from Streptococcus faecalis subsp. zymogenes.

2. Restriction enzymes: properties and use.

3. Identification of a new sequence-specific endonuclease, NgoII, from Neisseria gonorrhoeae.

4. Two unique restriction endonucleases from Neisseria lactamica.

5. Specific fragments of phi X174 deoxyribonucleic acid produced by a restriction enzyme from Haemophilus aegyptius, endonuclease Z.

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

7. Restriction enzymes and their isoschizomers.

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10. A restriction endonuclease SuaI from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius.