Literature DB >> 760866

Hemoglobin biosynthesis in individual bursts in culture: studies of human umbilical cord blood.

K Kidoguchi, M Ogawa, J D Karam, J S McNeil, M S Fitch.   

Abstract

We cultured human umbilical cord blood erythropoietic precursors in methyl cellulose clonal assay and analyzed the synthetic rates of Hb A and Hb F in individual erythropoietic bursts. Hemoglobin was labeled with 14C-amino acids in culture, separated by slab gel isoelectric focusing techniques, and quantitated by fluorographic methods. Almost all bursts exhibited both Hb A and Hb F in varying ratios. Frequencies of the individual bursts differing in percentage Hb F biosynthesis had normal distributions. Natal erythropoietic precursors appeared to be randomly committed to Hb F synthesis.

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Year:  1979        PMID： 760866

Source DB:  PubMed          Journal:  Blood        ISSN： 0006-4971            Impact factor:   22.113


  3 in total

1.  Investigations of the simian ontogenic switch from fetal to adult hemoglobin at the progenitor cell level.

Authors:  B P Alter; R S Weinberg; D C Linch; J M Schofield; B T Jackson; G J Piasecki; J C Thornton; D G Nathan
Journal:  J Clin Invest       Date:  1986-12       Impact factor: 14.808

2.  Globin chain synthesis in single erythroid bursts from cord blood: studies on gamma leads to beta and G gamma leads to A gamma switches.

Authors:  P Comi; B Giglioni; S Ottolenghi; A M Gianni; E Polli; P Barba; A Covelli; G Migliaccio; M Condorelli; C Peschle
Journal:  Proc Natl Acad Sci U S A       Date:  1980-01       Impact factor: 11.205

3.  Hemoglobin switching in culture: evidence for a humoral factor that induces switching in adult and neonatal but not fetal erythroid cells.

Authors:  T Papayannopoulou; S Kurachi; B Nakamoto; E D Zanjani; G Stamatoyannopoulos
Journal:  Proc Natl Acad Sci U S A       Date:  1982-11       Impact factor: 11.205
  3 in total

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