Literature DB >> 7606047

7-Deaza purine bases offer a higher ion stability in the analysis of DNA by matrix-assisted laser desorption/ionization mass spectrometry.

F Kirpekar1, E Nordhoff, K Kristiansen, P Roepstorff, S Hahner, F Hillenkamp.   

Abstract

Oligodeoxynucleotides which contain 7-deaza analogues of the normal purine nucleotides have been synthesized both enzymatically and chemically. When subjected to matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis, the modified samples offer both higher stability and increased sensitivity compared to otherwise identical unmodified oligodeoxynucleotides. In view of these observations, models for the fragmentation of oligodeoxynucleotides in MALDI-MS with positive ion detection mode are presented. Additionally, the potential use of 7-deaza purine nucleotides in the MALDI-MS analysis of DNA sequencing reactions is discussed.

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Year:  1995        PMID： 7606047     DOI： 10.1002/rcm.1290090611

Source DB:  PubMed          Journal:  Rapid Commun Mass Spectrom        ISSN： 0951-4198            Impact factor:   2.419


  14 in total

1.  Fragmentation pathway studies of oligonucleotides in matrix-assisted laser desorption/ionization mass spectrometry by charge tagging and H/D exchange.

Authors:  Chau-Wen Chou; Patrick A Limbach; Richard B Cole
Journal:  J Am Soc Mass Spectrom       Date:  2002-12       Impact factor: 3.109

Review 2.  MALDI-TOF mass spectrometry: a versatile tool for high-performance DNA analysis.

Authors:  Christian Jurinke; Paul Oeth; Dirk van den Boom
Journal:  Mol Biotechnol       Date:  2004-02       Impact factor: 2.695

3.  Mononucleotide gas-phase proton affinities as determined by the kinetic method.

Authors:  K B Green-Church; P A Limbach
Journal:  J Am Soc Mass Spectrom       Date:  2000-01       Impact factor: 3.109

4.  Leveling response factors in the electrospray ionization process using a heated capillary interface.

Authors:  Jennifer L Frahm; David C Muddiman; Michael J Burke
Journal:  J Am Soc Mass Spectrom       Date:  2005-05       Impact factor: 3.109

5.  RNA fragmentation in MALDI mass spectrometry studied by H/D-exchange: mechanisms of general applicability to nucleic acids.

Authors:  Thomas E Andersen; Finn Kirpekar; Kim F Haselmann
Journal:  J Am Soc Mass Spectrom       Date:  2006-07-27       Impact factor: 3.109

6.  DNA sequencing by delayed extraction-matrix-assisted laser desorption/ionization time of flight mass spectrometry.

Authors:  M T Roskey; P Juhasz; I P Smirnov; E J Takach; S A Martin; L A Haff
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-14       Impact factor: 11.205

7.  Investigations of the metastable decay of DNA under ultraviolet matrix-assisted laser desorption/ionization conditions with post-source-decay analysis and hydrogen/deuterium exchange.

Authors:  J Gross; A Leisner; F Hillenkamp; S Hahner; M Karas; J Schäfer; F Lützenkirchen; E Nordhoff
Journal:  J Am Soc Mass Spectrom       Date:  1998-09       Impact factor: 3.109

8.  DNA sequence analysis by MALDI mass spectrometry.

Authors:  F Kirpekar; E Nordhoff; L K Larsen; K Kristiansen; P Roepstorff; F Hillenkamp
Journal:  Nucleic Acids Res       Date:  1998-06-01       Impact factor: 16.971

9.  Structure and fragmentation mechanisms of isomeric T-rich oligodeoxynucleotides: a comparison of four tandem mass spectrometric methods.

Authors:  Z Wang; K X Wan; R Ramanathan; J S Taylor; M L Gross
Journal:  J Am Soc Mass Spectrom       Date:  1998-07       Impact factor: 3.109

10.  Sodium controlled selective reactivity of protonated deoxy-oligonucleotides in the gas phase.

Authors:  Helga Dögg Flosadóttir; Michal Stano; Oddur Ingólfsson
Journal:  J Am Soc Mass Spectrom       Date:  2008-12-24       Impact factor: 3.109
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