Literature DB >> 7599318

Successful expression in pollen of various plant species of in vitro synthesized mRNA introduced by particle bombardment.

T Tanaka1, M Nishihara, M Seki, A Sakamoto, K Tanaka, K Irifune, H Morikawa.   

Abstract

Gold particles coated with beta-glucuronidase (GUS) mRNA with a 5' cap structure that had been synthesized in vitro were introduced, by use of a pneumatic particle gun, into pollen grains of lily (Lilium longiflorum), freesia (Freesia refracta) and tulip (Tulipa gesneriana). A fluorometric assay for the GUS activity indicated that in vitro synthesized GUS mRNA introduced into these pollen cells by particle bombardment was successfully expressed. GUS activity in extracts of the bombarded lily pollen became detectable fluorometrically within 30 min after bombardment, peaked at 6 h, then gradually decreased. This activity changed as a function of the developmental stage of the pollen cell of lily.

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 7599318     DOI： 10.1007/BF00020252

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  9 in total

1.  Gene delivery into cultured plant cells by DNA-coated gold particles accelerated by a pneumatic particle gun.

Authors:  A Iida; M Seki; M Kamada; Y Yamada; H Morikawa
Journal:  Theor Appl Genet       Date:  1990-12       Impact factor: 5.699

2.  Visualizing mRNA expression in plant protoplasts: factors influencing efficient mRNA uptake and translation.

Authors:  D R Gallie; W J Lucas; V Walbot
Journal:  Plant Cell       Date:  1989-03       Impact factor: 11.277

3.  Three cDNA sequences coding for glutamine synthetase polypeptides in Oryza sativa L.

Authors:  A Sakamoto; M Ogawa; T Masumura; D Shibata; G Takeba; K Tanaka; S Fujii
Journal:  Plant Mol Biol       Date:  1989-11       Impact factor: 4.076

4.  Isolation and expression of an anther-specific gene from tomato.

Authors:  D Twell; R Wing; J Yamaguchi; S McCormick
Journal:  Mol Gen Genet       Date:  1989-06

5.  Evaluation of pollen viability by enzymatically induced fluorescence; intracellular hydrolysis of fluorescein diacetate.

Authors:  J Heslop-Harrison; Y Heslop-Harrison
Journal:  Stain Technol       Date:  1970-05

6.  Transient expression of chimeric genes delivered into pollen by microprojectile bombardment.

Authors:  D Twell; T M Klein; M E Fromm; S McCormick
Journal:  Plant Physiol       Date:  1989-12       Impact factor: 8.340

7.  Stable Transformation of Soybean Callus by DNA-Coated Gold Particles.

Authors:  P Christou; D E McCabe; W F Swain
Journal:  Plant Physiol       Date:  1988-07       Impact factor: 8.340

8.  Expression of the [beta]-Glucuronidase Gene in Pollen of Lily (Lilium longiflorum), Tobacco (Nicotiana tabacum), Nicotiana rustica, and Peony (Paeonia lactiflora) by Particle Bombardment.

Authors:  M. Nishihara; M. Ito; I. Tanaka; M. Kyo; K. Ono; K. Irifune; H. Morikawa
Journal:  Plant Physiol       Date:  1993-06       Impact factor: 8.340

9.  GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

Authors:  R A Jefferson; T A Kavanagh; M W Bevan
Journal:  EMBO J       Date:  1987-12-20       Impact factor: 11.598
  9 in total
  2 in total

Review 1.  Pneumonia.

Authors:  Antoni Torres; Catia Cilloniz; Michael S Niederman; Rosario Menéndez; James D Chalmers; Richard G Wunderink; Tom van der Poll
Journal:  Nat Rev Dis Primers       Date:  2021-04-08       Impact factor: 52.329

Review 2.  Translation in plants--rules and exceptions.

Authors:  J Fütterer; T Hohn
Journal:  Plant Mol Biol       Date:  1996-10       Impact factor: 4.076
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.