Evaluation of the single needle procedure in plateletpheresis with the fresenius AS 104 blood cell separator.

The aim of the present study was to evaluate the single needle (SN) procedure for plateletpheresis with the AS 104 blood cell separator. One hundred fifty-seven platelet collections with different protocols were carried out and analysed with respect to platelet yield, separation efficiency, leukocyte contamination, anticoagulant infused, and donation time. SN plateletpheresis resulted in a mean platelet yield of 2.6-3.1 x 10(11) with separation efficiencies of 49.2-57.4%. The mean leukocyte contamination ranged from 2.8 to 40.5 x 10(6). Parameters of product quality were investigated in 14 platelet concentrates (PC). Determination of glucose, lactate, lactate dehydrogenase (LDH), and morphology score showed good quality of the PC.