| Literature DB >> 7592360 |
D U Kloos1, A A DiMarco, D A Elsemore, K N Timmis, L N Ornston.
Abstract
Cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor Acinetobacter calcoaceticus DNA. A different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 kb. Ligation of unlinked chromosomal DNA fragments allowed them to be linked genetically through natural transformation.Entities:
Mesh:
Year: 1995 PMID: 7592360 PMCID: PMC177435 DOI: 10.1128/jb.177.20.6015-6017.1995
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490