Literature DB >> 7590311

pDblet, a stable autonomously replicating shuttle vector for Schizosaccharomyces pombe.

C Brun1, D D Dubey, J A Huberman.   

Abstract

We have constructed a new multipurpose stable shuttle vector for the <span class="Species">fission yeast Schizosaccharomyces pombe (Sp). Plasmid pDblet was designed to provide convenient features for molecular work and to overcome the inconveniences of previously designed Sp vectors. It contains the Sp ura4 gene as selectable marker and a new highly efficient ARS (autonomously replicating sequence) element, allowing the vector to remain stable as a monomer in Sp. In addition, pDblet transforms Sp with high efficiency and has high mitotic stability and low copy number.

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Year:  1995        PMID： 7590311     DOI： 10.1016/0378-1119(95)00497-t

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  25 in total

1.  Rad8Rad5/Mms2-Ubc13 ubiquitin ligase complex controls translesion synthesis in fission yeast.

Authors:  Stéphane Coulon; Sharada Ramasubramanyan; Carole Alies; Gaëlle Philippin; Alan Lehmann; Robert P Fuchs
Journal:  EMBO J       Date:  2010-05-07       Impact factor: 11.598

2.  The fission yeast synaptobrevin ortholog Syb1 plays an important role in forespore membrane formation and spore maturation.

Authors:  Tomomi Yamaoka; Kazuki Imada; Kana Fukunishi; Yuriko Yamasaki; Chikashi Shimoda; Taro Nakamura
Journal:  Eukaryot Cell       Date:  2013-05-24

3.  The Hsk1(Cdc7) replication kinase regulates origin efficiency.

Authors:  Prasanta K Patel; Naveen Kommajosyula; Adam Rosebrock; Aaron Bensimon; Janet Leatherwood; John Bechhoefer; Nicholas Rhind
Journal:  Mol Biol Cell       Date:  2008-09-17       Impact factor: 4.138

4.  Mutational effect of fission yeast polalpha on cell cycle events.

Authors:  D Bhaumik; T S Wang
Journal:  Mol Biol Cell       Date:  1998-08       Impact factor: 4.138

5.  Dominant genetic screen for cofactors that enhance antisense RNA-mediated gene silencing in fission yeast.

Authors:  Mitch Raponi; Greg M Arndt
Journal:  Nucleic Acids Res       Date:  2002-06-01       Impact factor: 16.971

6.  Pac1p, an RNase III homolog, is required for formation of the 3' end of U2 snRNA in Schizosaccharomyces pombe.

Authors:  D Zhou; D Frendewey; S M Lobo Ruppert
Journal:  RNA       Date:  1999-08       Impact factor: 4.942

7.  Disruption and overexpression of the Schizosaccharomyces pombe aph1 gene and the effects on intracellular diadenosine 5',5'''-P1, P4-tetraphosphate (Ap4A), ATP and ADP concentrations.

Authors:  S W Ingram; L D Barnes
Journal:  Biochem J       Date:  2000-09-15       Impact factor: 3.857

8.  Plasmid construction using recombination activity in the fission yeast Schizosaccharomyces pombe.

Authors:  Ayako Chino; Kenji Watanabe; Hisao Moriya
Journal:  PLoS One       Date:  2010-03-11       Impact factor: 3.240

9.  Intronic sequence elements impede exon ligation and trigger a discard pathway that yields functional telomerase RNA in fission yeast.

Authors:  Ram Kannan; Sean Hartnett; Rodger B Voelker; J Andrew Berglund; Jonathan P Staley; Peter Baumann
Journal:  Genes Dev       Date:  2013-03-06       Impact factor: 11.361

10.  The fission yeast spo14+ gene encoding a functional homologue of budding yeast Sec12 is required for the development of forespore membranes.

Authors:  Michiko Nakamura-Kubo; Taro Nakamura; Aiko Hirata; Chikashi Shimoda
Journal:  Mol Biol Cell       Date:  2003-03       Impact factor: 4.138
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