Literature DB >> 7590302

Cloning and sequence analysis of the gene encoding the DNA polymerase I from Mycobacterium tuberculosis.

P Huberts1, V Mizrahi.   

Abstract

The polA gene (encoding DNA polymerase I) from Mycobacterium tuberculosis was cloned using an internal gene segment probe generated by PCR amplification of genomic DNA [Mizrahi et al., Gene 136 (1993) 287-290]. The gene encodes a polypeptide 904 amino acids (aa) in length that shares 89% identity with a 911-aa homologue from Mycobacterium leprae. The polypeptide has all of the primary structural elements necessary for DNA polymerase and 5'-3' exonuclease activity, but lacks the motifs required for an associated 3'-5' exonuclease (proofreading) activity.

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Year:  1995        PMID： 7590302     DOI： 10.1016/0378-1119(95)00453-d

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  9 in total

1.  Deoxy- and dideoxynucleotide discrimination and identification of critical 5' nuclease domain residues of the DNA polymerase I from Mycobacterium tuberculosis.

Authors:  V Mizrahi; P Huberts
Journal:  Nucleic Acids Res       Date:  1996-12-15       Impact factor: 16.971

2.  Characterization of the cydAB-encoded cytochrome bd oxidase from Mycobacterium smegmatis.

Authors:  B D Kana; E A Weinstein; D Avarbock; S S Dawes; H Rubin; V Mizrahi
Journal:  J Bacteriol       Date:  2001-12       Impact factor: 3.490

3.  Biochemical Characterization of Mycobacterium smegmatis RnhC (MSMEG_4305), a Bifunctional Enzyme Composed of Autonomous N-Terminal Type I RNase H and C-Terminal Acid Phosphatase Domains.

Authors:  Agata Jacewicz; Stewart Shuman
Journal:  J Bacteriol       Date:  2015-05-18       Impact factor: 3.490

4.  Characterization of two genes encoding the Mycobacterium tuberculosis ribonucleotide reductase small subunit.

Authors:  F Yang; S C Curran; L S Li; D Avarbock; J D Graf; M M Chua; G Lu; J Salem; H Rubin
Journal:  J Bacteriol       Date:  1997-10       Impact factor: 3.490

5.  Mycobacterium smegmatis DinB2 misincorporates deoxyribonucleotides and ribonucleotides during templated synthesis and lesion bypass.

Authors:  Heather Ordonez; Stewart Shuman
Journal:  Nucleic Acids Res       Date:  2014-10-28       Impact factor: 16.971

6.  Division of labor among Mycobacterium smegmatis RNase H enzymes: RNase H1 activity of RnhA or RnhC is essential for growth whereas RnhB and RnhA guard against killing by hydrogen peroxide in stationary phase.

Authors:  Richa Gupta; Debashree Chatterjee; Michael S Glickman; Stewart Shuman
Journal:  Nucleic Acids Res       Date:  2016-11-28       Impact factor: 16.971

7.  Comprehensive Analysis and Comparison on the Codon Usage Pattern of Whole Mycobacterium tuberculosis Coding Genome from Different Area.

Authors:  Li Gun; Ren Yumiao; Pan Haixian; Zhang Liang
Journal:  Biomed Res Int       Date:  2018-05-08       Impact factor: 3.411

8.  Mycobacterial DNA polymerase I: activities and crystal structures of the POL domain as apoenzyme and in complex with a DNA primer-template and of the full-length FEN/EXO-POL enzyme.

Authors:  Shreya Ghosh; Yehuda Goldgur; Stewart Shuman
Journal:  Nucleic Acids Res       Date:  2020-04-06       Impact factor: 16.971

9.  Characterization of three mycobacterial DinB (DNA polymerase IV) paralogs highlights DinB2 as naturally adept at ribonucleotide incorporation.

Authors:  Heather Ordonez; Maria Loressa Uson; Stewart Shuman
Journal:  Nucleic Acids Res       Date:  2014-09-08       Impact factor: 16.971
  9 in total

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