J Catt1, C O'Neill. 1. Human Reproduction Unit, Royal North Shore Hospital, St. Leonards, New South Wales, Australia.
Abstract
OBJECTIVE: To determine the effect of sperm manipulation before intracytoplasmic sperm injection (ICSI) on fertilization rates. DESIGN: Three methods of sperm manipulation before direct ICSI were compared in two sibling oocyte trials. SETTING: In vitro fertilization unit within a teaching hospital. PATIENTS: Patients undergoing infertility treatment using ICSI. INTERVENTIONS: Oocytes were inseminated by ICSI. MAIN OUTCOME MEASURE: Fertilization rate. RESULTS: In the first trial, a standard manipulation technique gave a 67% fertilization rate compared with 64% with a minimal manipulation technique. In the second trial the standard technique gave a significantly greater fertilization rate compared with no prior manipulation (67% versus 45%). CONCLUSIONS: Manipulation of sperm before direct ICSI is not mandatory for fertilization but significantly improves the fertilization rate.
OBJECTIVE: To determine the effect of sperm manipulation before intracytoplasmic sperm injection (ICSI) on fertilization rates. DESIGN: Three methods of sperm manipulation before direct ICSI were compared in two sibling oocyte trials. SETTING: In vitro fertilization unit within a teaching hospital. PATIENTS: Patients undergoing infertility treatment using ICSI. INTERVENTIONS: Oocytes were inseminated by ICSI. MAIN OUTCOME MEASURE: Fertilization rate. RESULTS: In the first trial, a standard manipulation technique gave a 67% fertilization rate compared with 64% with a minimal manipulation technique. In the second trial the standard technique gave a significantly greater fertilization rate compared with no prior manipulation (67% versus 45%). CONCLUSIONS: Manipulation of sperm before direct ICSI is not mandatory for fertilization but significantly improves the fertilization rate.