Literature DB >> 7589505

Cloning, expression and characterization of human thioltransferase (glutaredoxin) in E. coli.

C A Chrestensen1, C B Eckman, D W Starke, J J Mieyal.   

Abstract

PCR primers were designed from the known amino acid (aa) sequence for human red blood cell thioltransferase (hRBC TTase) and the known cDNA sequence for pig liver TTase (82% homologous) and used to amplify thioltransferase from a pool of human brain cDNAs. The PCR product was inserted into the pKK233-2 expression vector. The DNA sequence of the insert agreed with the aa sequence. High level expression of the enzyme was accomplished in E. coli, and Western blot analysis confirmed its identity. Recombinant TTase displayed catalytic properties indistinguishable from natural hRBC TTase.

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 7589505     DOI： 10.1016/0014-5793(95)01066-n

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

1.  Role of Bim in diallyl trisulfide-induced cytotoxicity in human cancer cells.

Authors:  Byeong-Chel Lee; Bae-Hang Park; Seog-Young Kim; Yong J Lee
Journal:  J Cell Biochem       Date:  2011-01       Impact factor: 4.429

2.  The yeast Saccharomyces cerevisiae contains two glutaredoxin genes that are required for protection against reactive oxygen species.

Authors:  S Luikenhuis; G Perrone; I W Dawes; C M Grant
Journal:  Mol Biol Cell       Date:  1998-05       Impact factor: 4.138

3.  Levodopa deactivates enzymes that regulate thiol-disulfide homeostasis and promotes neuronal cell death: implications for therapy of Parkinson's disease.

Authors:  Elizabeth A Sabens; Anne M Distler; John J Mieyal
Journal:  Biochemistry       Date:  2010-03-30       Impact factor: 3.162

4.  Carbon source-dependent regulation of a second gene encoding glutaredoxin from the fission yeast Schizosaccharomyces pombe.

Authors:  Hong-Gyum Kim; Ji-Hyun Kim; Byung-Chul Kim; Eun-Hee Park; Chang-Jin Lim
Journal:  Mol Biol Rep       Date:  2005-03       Impact factor: 2.316

5.  Glutaredoxin deficiency exacerbates neurodegeneration in C. elegans models of Parkinson's disease.

Authors:  William M Johnson; Chen Yao; Sandra L Siedlak; Wenzhang Wang; Xiongwei Zhu; Guy A Caldwell; Amy L Wilson-Delfosse; John J Mieyal; Shu G Chen
Journal:  Hum Mol Genet       Date:  2014-10-29       Impact factor: 6.150

6.  Selective inactivation of glutaredoxin by sporidesmin and other epidithiopiperazinediones.

Authors:  Usha Srinivasan; Aveenash Bala; Shu-chuan Jao; David W Starke; T William Jordan; John J Mieyal
Journal:  Biochemistry       Date:  2006-07-25       Impact factor: 3.162

7.  Kinetic and mechanistic characterization and versatile catalytic properties of mammalian glutaredoxin 2: implications for intracellular roles.

Authors:  Molly M Gallogly; David W Starke; Amanda K Leonberg; Susan M English Ospina; John J Mieyal
Journal:  Biochemistry       Date:  2008-09-25       Impact factor: 3.162

8.  Thioltransferase (glutaredoxin) mediates recovery of motor neurons from excitotoxic mitochondrial injury.

Authors:  Rajappa S Kenchappa; Latha Diwakar; Michael R Boyd; Vijayalakshmi Ravindranath
Journal:  J Neurosci       Date:  2002-10-01       Impact factor: 6.167

9.  Redox modulation of endothelial nitric oxide synthase by glutaredoxin-1 through reversible oxidative post-translational modification.

Authors:  Chun-An Chen; Francesco De Pascali; Ariel Basye; Craig Hemann; Jay L Zweier
Journal:  Biochemistry       Date:  2013-09-11       Impact factor: 3.162
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.