Literature DB >> 7586029

Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae.

S Sakamoto1, G Tamura, K Ito, T Ishikawa, K Iwano, N Nishiya.   

Abstract

The cDNA encoding the endo-beta-1,4-glucanase (carboxymethylcellulase; CMCase-I) from Aspergillus kawachii IFO 4308 was cloned. Nucleotide-sequence analysis of the cloned cDNA insert showed a 717-bp open reading frame that encoded a protein of 239 amino-acid residues. The predicted amino-acid sequence of the mature protein had considerable homology with the protein sequence of the FI-CMCase of Aspergillus aculeatus. The cDNA was introduced into Saccharomyces cerevisiae. The expressed enzyme had carboxylmethylcellulase activity, identified by clear zones on a CMC-agar plate after Congo Red staining.

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Year:  1995        PMID： 7586029     DOI： 10.1007/BF00311212

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  19 in total

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Authors:  P Béguin
Journal:  Annu Rev Microbiol       Date:  1990       Impact factor: 15.500

2.  Nucleotide sequence of the cellobiohydrolase gene from Trichoderma viride.

Authors:  C Cheng; N Tsukagoshi; S Udaka
Journal:  Nucleic Acids Res       Date:  1990-09-25       Impact factor: 16.971

Review 3.  Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.

Authors:  N R Gilkes; B Henrissat; D G Kilburn; R C Miller; R A Warren
Journal:  Microbiol Rev       Date:  1991-06

4.  Three-dimensional structure of cellobiohydrolase II from Trichoderma reesei.

Authors:  J Rouvinen; T Bergfors; T Teeri; J K Knowles; T A Jones
Journal:  Science       Date:  1990-07-27       Impact factor: 47.728

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  New M13 vectors for cloning.

Authors:  J Messing
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

7.  Cloning and sequence analysis of a cDNA for cellulase (FI-CMCase) from Aspergillus aculeatus.

Authors:  T Ooi; A Shinmyo; H Okada; S Hara; T Ikenaka; S Murao; M Arai
Journal:  Curr Genet       Date:  1990-10       Impact factor: 3.886

8.  Cellulase families revealed by hydrophobic cluster analysis.

Authors:  B Henrissat; M Claeyssens; P Tomme; L Lemesle; J P Mornon
Journal:  Gene       Date:  1989-09-01       Impact factor: 3.688

9.  Studies on transformation of Escherichia coli with plasmids.

Authors:  D Hanahan
Journal:  J Mol Biol       Date:  1983-06-05       Impact factor: 5.469

10.  Improved production of chymosin in Aspergillus by expression as a glucoamylase-chymosin fusion.

Authors:  M Ward; L J Wilson; K H Kodama; M W Rey; R M Berka
Journal:  Biotechnology (N Y)       Date:  1990-05
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  7 in total

1.  Cloning and bioinformatics analysis of an endoglucanase gene (Aucel12A) from Aspergillus usamii and its functional expression in Pichia pastoris.

Authors:  Hongling Shi; Xin Yin; Minchen Wu; Cunduo Tang; Huimin Zhang; Jianfang Li
Journal:  J Ind Microbiol Biotechnol       Date:  2011-09-21       Impact factor: 3.346

2.  An endoglucanase, EglA, from the hyperthermophilic archaeon Pyrococcus furiosus hydrolyzes beta-1,4 bonds in mixed-linkage (1-->3),(1-->4)-beta-D-glucans and cellulose.

Authors:  M W Bauer; L E Driskill; W Callen; M A Snead; E J Mathur; R M Kelly
Journal:  J Bacteriol       Date:  1999-01       Impact factor: 3.490

3.  Molecular characterization and heterologous expression of the gene encoding a low-molecular-mass endoglucanase from Trichoderma reesei QM9414.

Authors:  H Okada; K Tada; T Sekiya; K Yokoyama; A Takahashi; H Tohda; H Kumagai; Y Morikawa
Journal:  Appl Environ Microbiol       Date:  1998-02       Impact factor: 4.792

Review 4.  Aspergillus enzymes involved in degradation of plant cell wall polysaccharides.

Authors:  R P de Vries; J Visser
Journal:  Microbiol Mol Biol Rev       Date:  2001-12       Impact factor: 11.056

5.  The family 42 carbohydrate-binding module of family 54 alpha-L-arabinofuranosidase specifically binds the arabinofuranose side chain of hemicellulose.

Authors:  Akimasa Miyanaga; Takuya Koseki; Yozo Miwa; Yuichiro Mese; Sachiko Nakamura; Atsushi Kuno; Jun Hirabayashi; Hiroshi Matsuzawa; Takayoshi Wakagi; Hirofumi Shoun; Shinya Fushinobu
Journal:  Biochem J       Date:  2006-11-01       Impact factor: 3.857

6.  Biochemical characterization, molecular cloning, and structural modeling of an interesting β-1,4-glucanase from Sclerotinia sclerotiorum.

Authors:  Haifa Chahed; Aymen Ezzine; Amine Ben Mlouka; Julie Hardouin; Thierry Jouenne; Mohamed Najib Marzouki
Journal:  Mol Biotechnol       Date:  2014-04       Impact factor: 2.695

7.  Cloning and expression of an endoglucanase gene from the thermotolerant fungus Aspergillus fumigatus DBiNU-1 in Kluyveromyces lactis.

Authors:  Budsayachat Rungrattanakasin; Siripong Premjet; Sudarat Thanonkeo; Preekamol Klanrit; Pornthap Thanonkeo
Journal:  Braz J Microbiol       Date:  2018-02-03       Impact factor: 2.476
  7 in total

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