Literature DB >> 7585959

The multidomain structure of Orc1p reveals similarity to regulators of DNA replication and transcriptional silencing.

S P Bell1, J Mitchell, J Leber, R Kobayashi, B Stillman.   

Abstract

The origin recognition complex (ORC) is a six protein assembly that binds S. cerevisiae origins of replication and directs DNA replication throughout the genome and transcriptional silencing at the yeast mating-type loci. Here we report the cloning of the genes encoding the 120 kDa (ORC1), 62 kDa (ORC3), and 56 kDa (ORC4) subunits of ORC and the reconstitution of the complete complex after expression of all six subunits in insect cells. Orc1p is related to Cdc6p and Cdc18p, which regulate DNA replication and mitosis, and to Sir3p, a regulator of transcriptional silencing. The N-terminal region of Orc1p is highly related to Sir3p, and studies of Orc1p/Sir3p chimeric proteins indicate that this domain is dedicated to the transcriptional silencing function of ORC.

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Year:  1995        PMID： 7585959     DOI： 10.1016/0092-8674(95)90096-9

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  107 in total

1.  ATP bound to the origin recognition complex is important for preRC formation.

Authors:  R D Klemm; S P Bell
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-17       Impact factor: 11.205

2.  Sir3-dependent assembly of supramolecular chromatin structures in vitro.

Authors:  P T Georgel; M A Palacios DeBeer; G Pietz; C A Fox; J C Hansen
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-10       Impact factor: 11.205

3.  Mechanisms controlling differential promoter-occupancy by the yeast forkhead proteins Fkh1p and Fkh2p: implications for regulating the cell cycle and differentiation.

Authors:  P C Hollenhorst; G Pietz; C A Fox
Journal:  Genes Dev       Date:  2001-09-15       Impact factor: 11.361

4.  Regulation of origin recognition complex conformation and ATPase activity: differential effects of single-stranded and double-stranded DNA binding.

Authors:  D G Lee; A M Makhov; R D Klemm; J D Griffith; S P Bell
Journal:  EMBO J       Date:  2000-09-01       Impact factor: 11.598

5.  The role of nucleotide binding and hydrolysis in the function of the fission yeast cdc18(+) gene product.

Authors:  D DeRyckere; C L Smith; G S Martin
Journal:  Genetics       Date:  1999-04       Impact factor: 4.562

6.  Two classes of sir3 mutants enhance the sir1 mutant mating defect and abolish telomeric silencing in Saccharomyces cerevisiae.

Authors:  E M Stone; C Reifsnyder; M McVey; B Gazo; L Pillus
Journal:  Genetics       Date:  2000-06       Impact factor: 4.562

7.  Transcriptional silencing functions of the yeast protein Orc1/Sir3 subfunctionalized after gene duplication.

Authors:  Meleah A Hickman; Laura N Rusche
Journal:  Proc Natl Acad Sci U S A       Date:  2010-10-25       Impact factor: 11.205

8.  Degradation of origin recognition complex large subunit by the anaphase-promoting complex in Drosophila.

Authors:  Marito Araki; Robin P Wharton; Zhanyun Tang; Hongtao Yu; Maki Asano
Journal:  EMBO J       Date:  2003-11-17       Impact factor: 11.598

9.  Elaboration, diversification and regulation of the Sir1 family of silencing proteins in Saccharomyces.

Authors:  Jennifer E G Gallagher; Joshua E Babiarz; Leonid Teytelman; Kenneth H Wolfe; Jasper Rine
Journal:  Genetics       Date:  2009-01-26       Impact factor: 4.562

10.  Structure and function of the Saccharomyces cerevisiae Sir3 BAH domain.

Authors:  Jessica J Connelly; Peihua Yuan; Hao-Chi Hsu; Zhizhong Li; Rui-Ming Xu; Rolf Sternglanz
Journal:  Mol Cell Biol       Date:  2006-04       Impact factor: 4.272
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