| Literature DB >> 7584047 |
H Suzuki1, J Kawai, C Taga, N Ozawa, S Watanabe.
Abstract
A PCR-mediated direct cloning for target spot DNA from RLGS gel has been established. The method consists of PCR amplification of adaptor-ligated spot DNA fragments without excluding similar-sized DNA fragments co-localized on RLGS gel, and following selective ligation with the NotI-dT vector. Applying this method, we have successfully cloned several DNA fragments derived from target spots whose intensities change developmentally due to DNA methylation in the telencephalon of C3H/HeN mice. Since only a few micrograms of total DNA is sufficient for our spot cloning, our method may be highly useful when the total DNA sample prepared for cloning is limited.Entities:
Mesh：
Substances：
Year: 1994 PMID： 7584047 DOI： 10.1093/dnares/1.5.245
Source DB: PubMed Journal: DNA Res ISSN： 1340-2838 Impact factor: 4.458