Literature DB >> 7583934

Bioactive tumor necrosis factor in the sputa of cystic fibrosis patients.

N Kelly1, S Clee, B Nakielna.   

Abstract

Eighty sputum samples from 37 cystic fibrosis patients were assayed for tumor necrosis factor alpha (TNF-alpha) in the L929 bioactivity assay. In addition, the presence of TNF-alpha-specific mRNA in the sputum was determined by using TNF-alpha-specific DNA probes in a PCR amplification step after reverse transcription of the mRNA to cDNA. Bioactive TNF-alpha was detected in 69 (86%) of the 80 sputum samples tested and, on at least one occasion, in 35 (95%) of the 37 cystic fibrosis patients sampled. The highest levels of TNF-alpha were observed in sputa obtained from patients presenting with an exacerbation of their disease.

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Year:  1995        PMID： 7583934      PMCID： PMC170189          DOI： 10.1128/cdli.2.4.499-502.1995

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  12 in total

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Authors:  E Levy; C Gurbindo; F Lacaille; K Paradis; L Thibault; E Seidman
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Authors:  P Greally; M J Hussein; A J Cook; A P Sampson; P J Piper; J F Price
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