PURPOSE: Sperm capacitation and the acrosome reaction are prerequisites to accomplishing fertilization. The object of this research was to induce capacitation and the acrosome reaction of human sperm, comparing the acrosome status of fertile and infertile men, using alpha-amylase. METHODS: Acrosome status was measured using indirect immunofluorescence (IIF; monoclonal antibody GB24, Thèramex), hemizona assay (HZA), and transmission electron microscopy (TEM). RESULTS: Acrosome-reacted sperm without alpha-amylase treatment was significantly more abundant in the fertile versus the infertile group utilizing IIF, HZA, and TEM. alpha-Amylase-treated spermatozoa from the fertile and infertile groups showed a significant increase in the number of sperm bound to the hemizona (P < 0.05). CONCLUSIONS: The percentage of acrosome-reacted sperm in the infertile group was significantly enhanced by alpha-amylase, utilizing IIF and TEM.
PURPOSE: Sperm capacitation and the acrosome reaction are prerequisites to accomplishing fertilization. The object of this research was to induce capacitation and the acrosome reaction of human sperm, comparing the acrosome status of fertile and infertile men, using alpha-amylase. METHODS: Acrosome status was measured using indirect immunofluorescence (IIF; monoclonal antibody GB24, Thèramex), hemizona assay (HZA), and transmission electron microscopy (TEM). RESULTS: Acrosome-reacted sperm without alpha-amylase treatment was significantly more abundant in the fertile versus the infertile group utilizing IIF, HZA, and TEM. alpha-Amylase-treated spermatozoa from the fertile and infertile groups showed a significant increase in the number of sperm bound to the hemizona (P < 0.05). CONCLUSIONS: The percentage of acrosome-reacted sperm in the infertile group was significantly enhanced by alpha-amylase, utilizing IIF and TEM.
Authors: J P Vermeiden; R E Bernardus; C S ten Brug; C H Statema-Lohmeijer; A M Willemsen-Brugma; J Schoemaker Journal: Fertil Steril Date: 1989-01 Impact factor: 7.329
Authors: D R Franken; S Oehninger; L J Burkman; C C Coddington; T F Kruger; Z Rosenwaks; A A Acosta; G D Hodgen Journal: J In Vitro Fert Embryo Transf Date: 1989-02
Authors: L Calvo; L Dennison-Lagos; S M Banks; A Dorfmann; L P Thorsell; M Bustillo; J D Schulman; R J Sherins Journal: Hum Reprod Date: 1994-10 Impact factor: 6.918
Authors: T F Kruger; R Menkveld; F S Stander; C J Lombard; J P Van der Merwe; J A van Zyl; K Smith Journal: Fertil Steril Date: 1986-12 Impact factor: 7.329