A N Wakim1, S L Polizotto, D R Burholt. 1. Department of Obstetrics/Gynecology, Medical College of Pennsylvania/ Allegheny Campus, Pittsburgh 15212, USA.
Abstract
PURPOSE: The objective of this study was to investigate the influence of thyroid hormone on estradiol and progesterone secretion of human granulosa cells maintained in vitro. METHODS: Granulosa cells were obtained by aspiration of preovulatory follicles of woman undergoing assisted reproductive technology. Ovulation induction was performed with GnRH agonist, hMG, and hCG. RESULTS: Granulosa cells were maintained in vitro in a defined medium with added insulin. Twenty-four-hour estradiol and progesterone secretion into the medium were determined for granulosa cells growing in serum-free medium and in serum-free medium with added T4 in a concentration range of 10(-7) to 10(-11) M. CONCLUSIONS: All concentrations of T4 used produced a statistically significant increase in progesterone secretion (range, 1.39 to 1.60 times the baseline amount). The increase in estradiol secretion reached statistical significance only at a T4 concentration of 10(-8) M (1.24 times the baseline amount).
PURPOSE: The objective of this study was to investigate the influence of thyroid hormone on estradiol and progesterone secretion of human granulosa cells maintained in vitro. METHODS: Granulosa cells were obtained by aspiration of preovulatory follicles of woman undergoing assisted reproductive technology. Ovulation induction was performed with GnRH agonist, hMG, and hCG. RESULTS: Granulosa cells were maintained in vitro in a defined medium with added insulin. Twenty-four-hour estradiol and progesterone secretion into the medium were determined for granulosa cells growing in serum-free medium and in serum-free medium with added T4 in a concentration range of 10(-7) to 10(-11) M. CONCLUSIONS: All concentrations of T4 used produced a statistically significant increase in progesterone secretion (range, 1.39 to 1.60 times the baseline amount). The increase in estradiol secretion reached statistical significance only at a T4 concentration of 10(-8) M (1.24 times the baseline amount).
Authors: D W Cramer; P M Sluss; R D Powers; P McShane; E S Ginsburgs; M D Hornstein; A F Vitonis; R L Barbieri Journal: J Assist Reprod Genet Date: 2003-06 Impact factor: 3.412
Authors: Lenka Bruckova; Tomas Soukup; Benjamin Visek; Jiri Moos; Martina Moosova; Jana Pavelkova; Karel Rezabek; Lenka Kucerova; Stanislav Micuda; Eva Brcakova; Jaroslav Mokry Journal: J Assist Reprod Genet Date: 2011-08-06 Impact factor: 3.412