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Literature DB >> 7579998

Dehalogenation of haloalkanes by Rhodococcus erythropolis Y2. The presence of an oxygenase-type dehalogenase activity complements that of an halidohydrolase activity.

S J Armfield¹, P J Sallis, P B Baker, A T Bull, D J Hardman.

Abstract

Rhodococcus erythropolis Y2 produced two types of dehalogenase: a hydrolytic enzyme, that is an halidohydrolase, which was induced by C3 to C6 1-haloalkane substrates, and at least one oxygenase-type dehalogenase induced by C7 to C16 1-haloalkanes and n-alkanes. The oxygenase-type activity dehalogenated C4 to C18 1-chloroalkanes with an optimum activity towards 1-chlorotetradecane. The halidohydrolase catalysed the dehalogenation of a wide range of 1- and alpha,omega-disubstituted haloalkanes and alpha,omega-substituted haloalcohols. In resting cell suspensions of hexadecane-grown R. erythropolis Y2 the oxygenase-type dehalogenase had a specific activity of 12.9 mU (mg protein)-1 towards 1-chlorotetradecane (3.67 mU mg-1 towards 1-chlorobutane) whereas the halidohydrolase in 1-chlorobutane-grown batch cultures had a specific activity of 44 mU (mg protein)-1 towards 1-chlorobutane. The significance of the two dehalogenase systems in a single bacterial strain is discussed in terms of their contribution to the overall catabolic potential of the organism.

Entities: Chemical Species

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Year: 1995 PMID： 7579998 DOI： 10.1007/BF00700463

Source DB: PubMed Journal: Biodegradation ISSN： 0923-9820 Impact factor: 3.909

27 in total

1. Poly-beta-hyroxybutyrate metabolism in washed suspensions of Bacillus cereus and Bacillus megaterium.

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2. Microbial oxidation of hydrocarbons and related compounds by whole-cell suspensions of the methane-oxidizing bacterium h-2.

Authors: T Imai; H Takigawa; S Nakagawa; G J Shen; T Kodama; Y Minoda
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3. Permeabilization of microorganisms by Triton X-100.

Authors: G F Miozzari; P Niederberger; R Hütter
Journal: Anal Biochem Date: 1978-10-01 Impact factor: 3.365

4. Enzymatic omega-oxidation. IV. Purification and properties of the omega-hydroxylase of Pseudomonas oleovorans.

Authors: E J McKenna; M J Coon
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5. A low-viscosity epoxy resin embedding medium for electron microscopy.

Authors: A R Spurr
Journal: J Ultrastruct Res Date: 1969-01

6. Purification and properties of haloalkane dehalogenase from Corynebacterium sp. strain m15-3.

Authors: T Yokota; T Omori; T Kodama
Journal: J Bacteriol Date: 1987-09 Impact factor: 3.490

Review 7. Biotransformation of halogenated compounds.

Authors: D J Hardman
Journal: Crit Rev Biotechnol Date: 1991 Impact factor: 8.429

8. The effect of toluene on the structure and permeability of the outer and cytoplasmic membranes of Escherichia coli.

Authors: M J de Smet; J Kingma; B Witholt
Journal: Biochim Biophys Acta Date: 1978-01-04

9. The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath). Its ability to oxygenate n-alkanes, n-alkenes, ethers, and alicyclic, aromatic and heterocyclic compounds.

Authors: J Colby; D I Stirling; H Dalton
Journal: Biochem J Date: 1977-08-01 Impact factor: 3.857

10. Cloning of 1,2-dichloroethane degradation genes of Xanthobacter autotrophicus GJ10 and expression and sequencing of the dhlA gene.

Authors: D B Janssen; F Pries; J van der Ploeg; B Kazemier; P Terpstra; B Witholt
Journal: J Bacteriol Date: 1989-12 Impact factor: 3.490

6 in total

Dehalogenation of haloalkanes by Rhodococcus erythropolis Y2. The presence of an oxygenase-type dehalogenase activity complements that of an halidohydrolase activity.

1. Poly-beta-hyroxybutyrate metabolism in washed suspensions of Bacillus cereus and Bacillus megaterium.

2. Microbial oxidation of hydrocarbons and related compounds by whole-cell suspensions of the methane-oxidizing bacterium h-2.

3. Permeabilization of microorganisms by Triton X-100.

4. Enzymatic omega-oxidation. IV. Purification and properties of the omega-hydroxylase of Pseudomonas oleovorans.

5. A low-viscosity epoxy resin embedding medium for electron microscopy.

6. Purification and properties of haloalkane dehalogenase from Corynebacterium sp. strain m15-3.

Review 7. Biotransformation of halogenated compounds.

8. The effect of toluene on the structure and permeability of the outer and cytoplasmic membranes of Escherichia coli.

9. The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath). Its ability to oxygenate n-alkanes, n-alkenes, ethers, and alicyclic, aromatic and heterocyclic compounds.

10. Cloning of 1,2-dichloroethane degradation genes of Xanthobacter autotrophicus GJ10 and expression and sequencing of the dhlA gene.

1. Isolation and characterization of 2,3-dichloro-1-propanol-degrading rhizobia.

2. Dehalogenation of haloalkanes by Mycobacterium tuberculosis H37Rv and other mycobacteria.

3. Monooxygenase-mediated 1,2-dichloroethane degradation by Pseudomonas sp. strain DCA1.

4. Pseudomonas sp. strain 273, an aerobic alpha, omega-dichloroalkaneDegrading bacterium.

5. Complete reductive dechlorination of 1,2-dichloropropane by anaerobic bacteria.

6. Biodegradation of chlorinated alkanes and their commercial mixtures by Pseudomonas sp. strain 273.