Literature DB >> 7579995

Use of haloacetate dehalogenase genes as selection markers for Escherichia coli and Pseudomonas vectors.

H Kawasaki1, H Kuriyama, K Tonomura.   

Abstract

The haloacetate dehalogenase gene, dehH2, cloned from Moraxella sp. strain B could be used a selection marker gene for vectors in Escherichia coli and Pseudomonas putida. Haloacetates, especially iodoacetate, inhibit the growth of some microorganisms. The dehH2 gene introduced into the cells conferred iodoacetate resistance on them. Therefore, E. coli and P. putida transformed with vectors marked with dehH2 could be easily selected on plates containing iodoacetate.

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Year:  1995        PMID： 7579995     DOI： 10.1007/BF00700459

Source DB:  PubMed          Journal:  Biodegradation        ISSN： 0923-9820            Impact factor:   3.909


  5 in total

1.  Estimation of glycollic acid.

Authors:  S DAGLEY; A RODGERS
Journal:  Biochim Biophys Acta       Date:  1953-12

2.  Lack of homology between two haloacetate dehalogenase genes encoded on a plasmid from Moraxella sp. strain B.

Authors:  H Kawasaki; K Tsuda; I Matsushita; K Tonomura
Journal:  J Gen Microbiol       Date:  1992-07

3.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

4.  Transformation of Pseudomonas putida and Escherichia coli with plasmid-linked drug-resistance factor DNA.

Authors:  A M Chakrabarty; J R Mylroie; D A Friello; J G Vacca
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205

5.  Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.

Authors:  G Ditta; S Stanfield; D Corbin; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205
  5 in total

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