Y J Huh1, D I Ahn, S J Kim. 1. Department of Bacteriology, Korean Institute of Tuberculosis, Korean National Tuberculosis Association, Seoul, Korea.
Abstract
OBJECTIVE: To establish the usefulness of DNA fingerprinting for the epidemiology of Mycobacterium tuberculosis isolated from Korean tuberculosis patients. METHODS: Comparison of restriction fragment length polymorphism (RFLP) patterns produced by southern hybridization of PvuII-digested chromosomal DNA. RESULTS: IS6110-associated banding patterns of 41 isolates varied considerably, containing 1-13 copies. The RFLP pattern of the epidemiologically related M. tuberculosis isolates was identical in 8 of 10 groups of close contact patients. No noticeable differences in RFLP were observed between drug-sensitive and drug-resistant isolates. IS1081-containing restriction fragment analysis of 52 isolates showed 6 different banding patterns, and the C type was found dominant in Korea. Identification of G type M. tuberculosis, which has a 8.0 kb IS1081-containing PvuII fragment, is unusual because it has been observed only in M. bovis BCG so far. CONCLUSIONS: IS6110 was a very useful tool for tracing the transmission route of tuberculosis; IS1081 was also useful for subdividing M. tuberculosis into several groups.
OBJECTIVE: To establish the usefulness of DNA fingerprinting for the epidemiology of Mycobacterium tuberculosis isolated from Korean tuberculosis patients. METHODS: Comparison of restriction fragment length polymorphism (RFLP) patterns produced by southern hybridization of PvuII-digested chromosomal DNA. RESULTS: IS6110-associated banding patterns of 41 isolates varied considerably, containing 1-13 copies. The RFLP pattern of the epidemiologically related M. tuberculosis isolates was identical in 8 of 10 groups of close contact patients. No noticeable differences in RFLP were observed between drug-sensitive and drug-resistant isolates. IS1081-containing restriction fragment analysis of 52 isolates showed 6 different banding patterns, and the C type was found dominant in Korea. Identification of G type M. tuberculosis, which has a 8.0 kb IS1081-containing PvuII fragment, is unusual because it has been observed only in M. bovis BCG so far. CONCLUSIONS: IS6110 was a very useful tool for tracing the transmission route of tuberculosis; IS1081 was also useful for subdividing M. tuberculosis into several groups.
Authors: C N Classen; R Warren; M Richardson; J H Hauman; R P Gie; J H Ellis; P D van Helden; N Beyers Journal: Thorax Date: 1999-02 Impact factor: 9.139
Authors: Go Eun Choi; Mi Hee Jang; Eun Ju Song; Seok Hoon Jeong; Jae-Seok Kim; Wee Gyo Lee; Young Uh; Kyoung Ho Roh; Hye Soo Lee; Jong Hee Shin; Nam Hee Ryoo; Young Ree Kim; Joseph Jeong; Jee Hee Kim; Sun Min Lee; Jongyoun Yi; Sang Hyun Hwang; Hyung Hoi Kim; Eun Yup Lee; Chulhun L Chang; Moon-Bum Kim; Yeong Dae Kim Journal: J Korean Med Sci Date: 2010-11-24 Impact factor: 2.153