Literature DB >> 7578220

Does the sulfhydryl or the adenine moiety of CoA enhance firefly luciferase activity?

S R Ford1, L M Buck, F R Leach.   

Abstract

Light production by firefly luciferase is limited by product release resulting in flash kinetics. Several compounds (CoA, PPi, and nucleotides) transform the flash-form of light production into continuous light production. The sulfhydryl group of CoA is required; however, since nucleotides are also active, at least two mechanisms (sites) must exist.

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Year:  1995        PMID： 7578220     DOI： 10.1016/0167-4838(95)00150-s

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  4 in total

1.  Identification of a peroxisomal ATP carrier required for medium-chain fatty acid beta-oxidation and normal peroxisome proliferation in Saccharomyces cerevisiae.

Authors:  C W van Roermund; R Drissen; M van Den Berg; L Ijlst; E H Hettema; H F Tabak; H R Waterham; R J Wanders
Journal:  Mol Cell Biol       Date:  2001-07       Impact factor: 4.272

2.  Firefly luciferase has two nucleotide binding sites: effect of nucleoside monophosphate and CoA on the light-emission spectra.

Authors:  J P Steghens; K L Min; J C Bernengo
Journal:  Biochem J       Date:  1998-11-15       Impact factor: 3.857

3.  Optimized bioluminescence analysis of adenosine triphosphate (ATP) released by platelets and its application in the high throughput screening of platelet inhibitors.

Authors:  Lili Wang; Yunqian Li; Ran Guo; Shanshan Li; Anqi Chang; Zhixiang Zhu; Pengfei Tu
Journal:  PLoS One       Date:  2019-10-10       Impact factor: 3.240

4.  Optical imaging of luminescence for in vivo quantification of gene electrotransfer in mouse muscle and knee.

Authors:  C Bloquel; C Trollet; E Pradines; J Seguin; D Scherman; M F Bureau
Journal:  BMC Biotechnol       Date:  2006-03-08       Impact factor: 2.563
  4 in total

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