PURPOSE: We characterize an undescribed antigen on prostatic epithelial cells, which is recognized by anti-CD38. MATERIALS AND METHODS: Normal (3 cases), benign hyperplastic (10) and carcinomatous (10) prostatic tissues were analyzed using immunohistochemistry, immuno-electron microscopy and the Western blot test. RESULTS: Comparison of prostatic and lymphatic CD38 antigen revealed identical bands at 45 kD. Electron microscopy demonstrated anti-CD38 reactivity on the cytoplasmic membrane and within the secretory vacuoles. Double labeling with anti-cytokeratin types 5/15 and 8/18 confirmed that basal and secretory normal prostatic epithelial cells express CD38. By contrast, a complete loss was found in malignant (52.8%), tumor-surrounding nonmalignant (16.3%) and benign prostatic hyperplasia derived glands (3.7%). CONCLUSIONS: CD38 is a novel prostatic antigen. Its role in intracellular calcium mobilization may contribute to smooth muscle cell contraction and/or sperm motility.
PURPOSE: We characterize an undescribed antigen on prostatic epithelial cells, which is recognized by anti-CD38. MATERIALS AND METHODS: Normal (3 cases), benign hyperplastic (10) and carcinomatous (10) prostatic tissues were analyzed using immunohistochemistry, immuno-electron microscopy and the Western blot test. RESULTS: Comparison of prostatic and lymphatic CD38 antigen revealed identical bands at 45 kD. Electron microscopy demonstrated anti-CD38 reactivity on the cytoplasmic membrane and within the secretory vacuoles. Double labeling with anti-cytokeratin types 5/15 and 8/18 confirmed that basal and secretory normal prostatic epithelial cells express CD38. By contrast, a complete loss was found in malignant (52.8%), tumor-surrounding nonmalignant (16.3%) and benign prostatic hyperplasia derived glands (3.7%). CONCLUSIONS:CD38 is a novel prostatic antigen. Its role in intracellular calcium mobilization may contribute to smooth muscle cell contraction and/or sperm motility.
Authors: Claudia C S Chini; Anatilde M Gonzalez Guerrico; Veronica Nin; Juliana Camacho-Pereira; Carlos Escande; Maria Thereza Barbosa; Eduardo N Chini Journal: Clin Cancer Res Date: 2013-09-11 Impact factor: 12.531
Authors: Jeffrey P Chmielewski; Sarah C Bowlby; Frances B Wheeler; Lihong Shi; Guangchao Sui; Amanda L Davis; Timothy D Howard; Ralph B D'Agostino; Lance D Miller; S Joseph Sirintrapun; Scott D Cramer; Steven J Kridel Journal: Mol Cancer Res Date: 2018-08-03 Impact factor: 5.852
Authors: Robert O Stuart; William Wachsman; Charles C Berry; Jessica Wang-Rodriguez; Linda Wasserman; Igor Klacansky; Dan Masys; Karen Arden; Steven Goodison; Michael McClelland; Yipeng Wang; Anne Sawyers; Iveta Kalcheva; David Tarin; Dan Mercola Journal: Proc Natl Acad Sci U S A Date: 2004-01-13 Impact factor: 11.205