Distribution of neurotensin-immunoreactive neurons in the digestive tract of the chicken.

The origin of the neurotensin-containing nerve fibers in the digestive tract of the chicken has been investigated with the use of colchicine and immunohistochemistry. Neurotensin-immunoreactive nerve fibers were found in the smooth muscle layers from the esophagus to the duodenum. Their density of distribution was very high in the esophagus and crop (maximum mean value: 1315/mm2 of sectional area in the lamina muscularis mucosae of the crop) and decreased progressively to the duodenum. Neurotensin-immunoreactive neuronal cell bodies were observed after colchicine treatment in the submucosal plexuses of the esophagus and crop and in the myenteric plexuses of the esophagus, crop, proventriculus and gizzard, and they extended varicose fibers. The number of neurotensin-immunoreactive cell bodies was high in the myenteric plexus of the gizzard (28.3 +/- 2.7/ganglion) but low in the plexuses of the esophagus, crop and proventriculus. Seven days after cutting the glossopharyngeal nerve and vagus nerve unilaterally, the number and extent of neurotensin-immunostained structures in the smooth muscle layers from the esophagus to the gizzard did not show any significant difference between operated and unoperated sides. These results indicate that in the chicken the great majority of neurotensin-immunoreactive enteric fibers originate in the intramural plexuses of the upper digestive wall and are mainly distributed to smooth muscle cells.