Literature DB >> 7559382

Purification and characterization of the XPF-ERCC1 complex of human DNA repair excision nuclease.

C H Park1, T Bessho, T Matsunaga, A Sancar.   

Abstract

A complex, which consists of ERCC1 (38 kDa) and a 112-kDa protein, was purified from HeLa cells to homogeneity. This complex complemented the nucleotide excision repair defects of rodent ERCC-1, ERCC-4, and human XP-F mutant cell-free extracts, indicating that the 112-kDa protein is XPF/ERCC4 and providing direct biochemical evidence that XPF and ERCC4 are identical. The XPF/ERCC4-ERCC1 complex has an endonuclease activity with preference for single-stranded DNA and a single-stranded region of duplex DNA with a "bubble" structure. This complex also nicks supercoiled DNA weakly, and this nicking activity is stimulated by human replication protein A when the DNA contains UV damage.

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Year:  1995        PMID： 7559382     DOI： 10.1074/jbc.270.39.22657

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  37 in total

Review 1.  Orchestrating the nucleases involved in DNA interstrand cross-link (ICL) repair.

Authors:  Blanka Sengerová; Anderson T Wang; Peter J McHugh
Journal:  Cell Cycle       Date:  2011-12-01       Impact factor: 4.534

2.  Crystal structure and DNA binding functions of ERCC1, a subunit of the DNA structure-specific endonuclease XPF-ERCC1.

Authors:  Oleg V Tsodikov; Jacquelin H Enzlin; Orlando D Schärer; Tom Ellenberger
Journal:  Proc Natl Acad Sci U S A       Date:  2005-08-02       Impact factor: 11.205

Review 3.  Other proteins interacting with XP proteins.

Authors:  Steven M Shell; Yue Zou
Journal:  Adv Exp Med Biol       Date:  2008       Impact factor: 2.622

4.  TFIIH-mediated nucleotide excision repair and initiation of mRNA transcription in an optimized cell-free DNA repair and RNA transcription assay.

Authors:  M S Satoh; P C Hanawalt
Journal:  Nucleic Acids Res       Date:  1996-09-15       Impact factor: 16.971

5.  Involvement of the Ras/extracellular signal-regulated kinase signalling pathway in the regulation of ERCC-1 mRNA levels by insulin.

Authors:  W Lee-Kwon; D Park; M Bernier
Journal:  Biochem J       Date:  1998-04-15       Impact factor: 3.857

Review 6.  Damage control: the pleiotropy of DNA repair genes in Drosophila melanogaster.

Authors:  J J Sekelsky; K C Burtis; R S Hawley
Journal:  Genetics       Date:  1998-04       Impact factor: 4.562

7.  Single-stranded DNA Binding by the Helix-Hairpin-Helix Domain of XPF Protein Contributes to the Substrate Specificity of the ERCC1-XPF Protein Complex.

Authors:  Devashish Das; Maryam Faridounnia; Lidija Kovacic; Robert Kaptein; Rolf Boelens; Gert E Folkers
Journal:  J Biol Chem       Date:  2016-12-27       Impact factor: 5.157

8.  Mapping of interaction domains between human repair proteins ERCC1 and XPF.

Authors:  W L de Laat; A M Sijbers; H Odijk; N G Jaspers; J H Hoeijmakers
Journal:  Nucleic Acids Res       Date:  1998-09-15       Impact factor: 16.971

9.  Effects on NaeI-DNA recognition of the leucine to lysine substitution that transforms restriction endonuclease NaeI to a topoisomerase: a model for restriction endonuclease evolution.

Authors:  K Jo; M D Topal
Journal:  Nucleic Acids Res       Date:  1996-11-01       Impact factor: 16.971

10.  Mutational analysis of the human nucleotide excision repair gene ERCC1.

Authors:  A M Sijbers; P J van der Spek; H Odijk; J van den Berg; M van Duin; A Westerveld; N G Jaspers; D Bootsma; J H Hoeijmakers
Journal:  Nucleic Acids Res       Date:  1996-09-01       Impact factor: 16.971
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