BACKGROUND & AIMS: The relationship between gastrin and the development of colorectal carcinoma (CRC) remains controversial. Problems with previous studies include failure to measure all forms of gastrin, lack of comparison between stored and secreted gastrin, and not controlling for Helicobacter pylori infection (a known cause of hypergastrinemia). The aim of this study was to quantify progastrin and progastrin-derived peptides in the resected tumor and plasma of patients with CRC and in the antrum and plasma of normal subjects. METHODS: Four region-specific gastrin antisera were used to measure progastrin, glycine-extended gastrin, amidated gastrin, and total gastrin. RESULTS: Progastrin, amidated gastrin, total gastrin, and glycine-extended gastrin were detected in 100%, 69%, 56%, and 44% of tumors, respectively (n = 32). When allowing for H. pylori infection, circulating amidated gastrin levels were not significantly elevated in patients with CRC. However, compared with control H. pylori-positive and H. pylori-negative subjects, fasting plasma total gastrin levels were increased in H. pylori-positive (5.2-fold) and H. pylori-negative (2.3-fold) patients with CRC. CONCLUSIONS: Gastrin or its processing intermediates are present in a high proportion of CRCs. Nonamidated gastrin levels are elevated in the circulation of patients with CRC regardless of H. pylori status. We conclude that gastrin should continue to be assessed as a circulating or autocrine growth factor in the development of CRC.
BACKGROUND & AIMS: The relationship between gastrin and the development of colorectal carcinoma (CRC) remains controversial. Problems with previous studies include failure to measure all forms of gastrin, lack of comparison between stored and secreted gastrin, and not controlling for Helicobacter pylori infection (a known cause of hypergastrinemia). The aim of this study was to quantify progastrin and progastrin-derived peptides in the resected tumor and plasma of patients with CRC and in the antrum and plasma of normal subjects. METHODS: Four region-specific gastrin antisera were used to measure progastrin, glycine-extended gastrin, amidated gastrin, and total gastrin. RESULTS: Progastrin, amidated gastrin, total gastrin, and glycine-extended gastrin were detected in 100%, 69%, 56%, and 44% of tumors, respectively (n = 32). When allowing for H. pyloriinfection, circulating amidated gastrin levels were not significantly elevated in patients with CRC. However, compared with control H. pylori-positive and H. pylori-negative subjects, fasting plasma total gastrin levels were increased in H. pylori-positive (5.2-fold) and H. pylori-negative (2.3-fold) patients with CRC. CONCLUSIONS:Gastrin or its processing intermediates are present in a high proportion of CRCs. Nonamidated gastrin levels are elevated in the circulation of patients with CRC regardless of H. pylori status. We conclude that gastrin should continue to be assessed as a circulating or autocrine growth factor in the development of CRC.
Authors: Lin Xiao; Suzana Kovac; Mike Chang; Arthur Shulkes; Graham S Baldwin; Oneel Patel Journal: Endocrinology Date: 2012-05-16 Impact factor: 4.736
Authors: David A Westwood; Oneel Patel; Christopher Christophi; Arthur Shulkes; Graham S Baldwin Journal: Int J Colorectal Dis Date: 2017-04-21 Impact factor: 2.571
Authors: Adeyinka O Laiyemo; Farin Kamangar; Pamela M Marcus; Philip R Taylor; Jarmo Virtamo; Demetrius Albanes; Rachael Z Stolzenberg-Solomon Journal: Cancer Causes Control Date: 2009-10-17 Impact factor: 2.506
Authors: Carrie A Duckworth; Daniel Clyde; Daniel L Worthley; Timothy C Wang; Andrea Varro; D Mark Pritchard Journal: Gastroenterology Date: 2013-03-19 Impact factor: 22.682