| Literature DB >> 7546700 |
Abstract
A nonradioactive functional assay was developed to quantitate DNA binding proteins. The assay was designed to allow the use of 96-well microplates for high sample throughput. We show that the assay can measure sequence-specific DNA binding of purified proteins as well as DNA binding activity present in whole cell extracts. By using a temperature-sensitive DNA binding protein, we demonstrate that DNA binding as measured by this novel assay correlates with the biological function of the protein.Entities:
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Year: 1995 PMID： 7546700
Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993