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Literature DB >> 7545052

A method for quantification and correction of proteins after transfer to immobilization membranes.

Abstract

A method is described for quantification of specific proteins after electrophoresis and transfer to immobilization membranes for Western blots. This method is analogous to methods used to correct the amounts of specific transcripts detected on Northern blots. Ponceau S staining of proteins bound to immobilization membranes is efficient and accurate compared to antibody binding in terms of time, effort and cost. Comparison of Ponceau S to other detection and staining methods for quantifying proteins and the basic chemistry of Ponceau S are summarized.

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Year: 1995 PMID： 7545052

Source DB: PubMed Journal: Biochem Mol Biol Int ISSN： 1039-9712

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Cited

34 in total

1. Sex differences in epigenetic regulation of the estrogen receptor-alpha promoter within the developing preoptic area.

Authors: Joseph R Kurian; Kristin M Olesen; Anthony P Auger
Journal: Endocrinology Date: 2010-03-17 Impact factor: 4.736

2. Ras mutation impairs epithelial barrier function to a wide range of nonelectrolytes.

Authors: James M Mullin; James M Leatherman; Mary Carmen Valenzano; Erika Rendon Huerta; Jon Verrechio; David M Smith; Karen Snetselaar; Mantao Liu; Mary Kay Francis; Christian Sell
Journal: Mol Biol Cell Date: 2005-09-21 Impact factor: 4.138

3. Pterostilbene reduces oxidative stress, prevents hypertrophy and preserves systolic function of right ventricle in cor pulmonale model.

Authors: Denise Dos Santos Lacerda; Patrick Türck; Bruna Gazzi de Lima-Seolin; Rafael Colombo; Vanessa Duarte Ortiz; Jéssica Hellen Poletto Bonetto; Cristina Campos-Carraro; Sara Elis Bianchi; Adriane Belló-Klein; Valquiria Linck Bassani; Alex Sander da Rosa Araujo
Journal: Br J Pharmacol Date: 2017-08-14 Impact factor: 8.739

A method for quantification and correction of proteins after transfer to immobilization membranes.

1. Sex differences in epigenetic regulation of the estrogen receptor-alpha promoter within the developing preoptic area.

2. Ras mutation impairs epithelial barrier function to a wide range of nonelectrolytes.

3. Pterostilbene reduces oxidative stress, prevents hypertrophy and preserves systolic function of right ventricle in cor pulmonale model.

4. Total protein is an effective loading control for cerebrospinal fluid western blots.

5. The aqueous phase of Alzheimer's disease brain contains assemblies built from ∼4 and ∼7 kDa Aβ species.

6. Muscle precursor cells isolated from aged rats exhibit an increased tumor necrosis factor- alpha response.

7. Age-dependent FOXO regulation of p27Kip1 expression via a conserved binding motif in rat muscle precursor cells.

8. Fibroblast growth factor 2-stimulated proliferation is lower in muscle precursor cells from old rats.

9. Caspase-9 regulates apoptosis/proliferation balance during metamorphic brain remodeling in Xenopus.

10. Early loss of cardiac function in acute myocardial infarction is associated with redox imbalance.