Literature DB >> 7542757

Localization of 12-lipoxygenase mRNA in cultured oligodendrocytes and astrocytes by in situ reverse transcriptase and polymerase chain reaction.

M K Bendani1, O Palluy, J Cook-Moreau, J L Beneytout, M Rigaud, J M Vallat.   

Abstract

We studied the distribution of 12-lipoxygenase mRNA in glial cells. First, mRNA was detected from cellular extracts by soluble-phase reverse transcriptase-polymerase chain reaction (RT-PCR). Taking into account that cell culture populations could not be 100% homogeneous, we then developed, for the first time, an in situ RT-PCR combined with immunocytochemistry with cell specific markers. Using this procedure we showed that 12-lipoxygenase mRNA was expressed both in mature oligodendrocytes and astrocytes.

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Year:  1995        PMID： 7542757     DOI： 10.1016/0304-3940(95)11482-c

Source DB:  PubMed          Journal:  Neurosci Lett        ISSN： 0304-3940            Impact factor:   3.046


  11 in total

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10.  Inhibition of 12/15-lipoxygenase by baicalein induces microglia PPARβ/δ: a potential therapeutic role for CNS autoimmune disease.

Authors:  J Xu; Y Zhang; Y Xiao; S Ma; Q Liu; S Dang; M Jin; Y Shi; B Wan; Y Zhang
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