| Literature DB >> 7542264 |
H C Smith-Vaughan1, K S Sriprakash, J D Mathews, D J Kemp.
Abstract
PCR-ribotyping, a new typing method based on long PCR, has been developed for nontypeable Haemophilus influenzae (NTHi). Ribosomal operons of NTHi were amplified by long PCR and were found to be highly polymorphic for internal HaeIII sites. The technique was applied to 49 isolates previously subjected to conventional ribotyping, and the two methods showed a high level of concordance for serial isolates from individual subjects. PCR-ribotyping provides a powerful new typing tool for strain characterization in epidemiological investigations of NTHi.Mesh:
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Year: 1995 PMID: 7542264 PMCID: PMC228129 DOI: 10.1128/jcm.33.5.1192-1195.1995
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948