Literature DB >> 7542264

Long PCR-ribotyping of nontypeable Haemophilus influenzae.

H C Smith-Vaughan1, K S Sriprakash, J D Mathews, D J Kemp.   

Abstract

PCR-ribotyping, a new typing method based on long PCR, has been developed for nontypeable Haemophilus influenzae (NTHi). Ribosomal operons of NTHi were amplified by long PCR and were found to be highly polymorphic for internal HaeIII sites. The technique was applied to 49 isolates previously subjected to conventional ribotyping, and the two methods showed a high level of concordance for serial isolates from individual subjects. PCR-ribotyping provides a powerful new typing tool for strain characterization in epidemiological investigations of NTHi.

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Year:  1995        PMID: 7542264      PMCID: PMC228129          DOI: 10.1128/jcm.33.5.1192-1195.1995

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  13 in total

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  22 in total

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