Literature DB >> 7542104

Characterization of cord-blood-derived human mast cells cultured in the presence of Steel factor and interleukin-6.

H Saito1, M Ebisawa, N Sakaguchi, T Onda, Y Iikura, M Yanagida, H Uzumaki, T Nakahata.   

Abstract

We generated > 10(7) mast cells by culturing 10(7) cord blood mononuclear cells for > 10 weeks in the presence of Steel factor, interleukin-6 and prostaglandin E2. 99% of the cultured cells had tryptase-positive granules, while 18% had chymase-positive granules. Cultured mast cells contained 3.6 micrograms histamine and 3.5 micrograms tryptase per 10(6) cells. Cells sensitized with 1 microgram/ml human IgE released 58.5% histamine and 1.55 ng tumor necrosis factor (TNF)-alpha per 10(6) cells when challenged with 1 microgram/ml antihuman IgE, whereas the control cells spontaneously released 3.7% histamine and 0.18 ng TNF-alpha. Analysis for surface antigens revealed that cultured mast cells expressed the following CD molecules: 9, 13, 14, 29, 33, 38, 43, 44, 45RA, 45RB, 46, 47, 48, 49d, 50, 51, 53, 54, 55, 58, 59, 60, 61 and 117 (c-Kit). Taken together, these cultured cells seem to be functionally mature mast cells.

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Year:  1995        PMID： 7542104     DOI： 10.1159/000236932

Source DB:  PubMed          Journal:  Int Arch Allergy Immunol        ISSN： 1018-2438            Impact factor:   2.749


  9 in total

1.  Interferon-gamma promotes the survival and Fc epsilon RI-mediated histamine release in cultured human mast cells.

Authors:  M Yanagida; H Fukamachi; M Takei; T Hagiwara; H Uzumaki; T Tokiwa; H Saito; Y Iikura; T Nakahata
Journal:  Immunology       Date:  1996-12       Impact factor: 7.397

2.  Inhibition of c-kit tyrosine kinase by imatinib mesylate induces apoptosis in mast cells in rheumatoid synovia: a potential approach to the treatment of arthritis.

Authors:  A Juurikivi; C Sandler; K A Lindstedt; P T Kovanen; T Juutilainen; M J Leskinen; T Mäki; K K Eklund
Journal:  Ann Rheum Dis       Date:  2005-08       Impact factor: 19.103

3.  Cultured basophils but not cultured mast cells induce human IgE synthesis in B cells after immunologic stimulation.

Authors:  Y Yanagihara; K Kajiwara; Y Basaki; K Ikizawa; M Ebisawa; C Ra; H Tachimoto; H Saito
Journal:  Clin Exp Immunol       Date:  1998-01       Impact factor: 4.330

4.  ER-27319, an acridone-related compound, inhibits release of antigen-induced allergic mediators from mast cells by selective inhibition of fcepsilon receptor I-mediated activation of Syk.

Authors:  K Moriya; J Rivera; S Odom; Y Sakuma; K Muramato; T Yoshiuchi; M Miyamoto; K Yamada
Journal:  Proc Natl Acad Sci U S A       Date:  1997-11-11       Impact factor: 11.205

5.  KIT signaling is dispensable for human mast cell progenitor development.

Authors:  Joakim S Dahlin; Maria Ekoff; Jennine Grootens; Liza Löf; Rose-Marie Amini; Hans Hagberg; Johanna S Ungerstedt; Ulla Olsson-Strömberg; Gunnar Nilsson
Journal:  Blood       Date:  2017-08-08       Impact factor: 22.113

6.  Stem cell factor expressed in human gastric mucosa in relation to mast cell increase in Helicobacter pylori-infected gastritis.

Authors:  Nobumichi Bamba; Shigemi Nakajima; Akira Andoh; Masamichi Bamba; Hiroyuki Sugihara; Tadao Bamba; Takanori Hattori
Journal:  Dig Dis Sci       Date:  2002-02       Impact factor: 3.199

7.  Tissue inhibitor of metalloproteinases 4 (TIMP4) is involved in inflammatory processes of human cardiovascular pathology.

Authors:  Ilpo Koskivirta; Otto Rahkonen; Mikko Mäyränpää; Sari Pakkanen; Michael Husheem; Annele Sainio; Harri Hakovirta; Jukka Laine; Eero Jokinen; Eero Vuorio; Petri Kovanen; Hannu Järveläinen
Journal:  Histochem Cell Biol       Date:  2006-03-07       Impact factor: 4.304

8.  Anti-apoptotic BFL-1 is the major effector in activation-induced human mast cell survival.

Authors:  Maria Ekoff; Katarina Lyberg; Maryla Krajewska; Monica Arvidsson; Sabina Rak; John C Reed; Ilkka Harvima; Gunnar Nilsson
Journal:  PLoS One       Date:  2012-06-15       Impact factor: 3.240

9.  Long-term treatment with imatinib results in profound mast cell deficiency in Ph+ chronic myeloid leukemia.

Authors:  Sabine Cerny-Reiterer; Anja Rabenhorst; Gabriele Stefanzl; Susanne Herndlhofer; Gregor Hoermann; Leonhard Müllauer; Sigrid Baumgartner; Christine Beham-Schmid; Wolfgang R Sperr; Christine Mannhalter; Heinz Sill; Werner Linkesch; Michel Arock; Karin Hartmann; Peter Valent
Journal:  Oncotarget       Date:  2015-02-20
  9 in total

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