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Literature DB >> 7542035

Single channel behavior of recombinant beta 2 gap junction connexons reconstituted into planar lipid bilayers.

L K Buehler¹, K A Stauffer, N B Gilula, N M Kumar.

Abstract

The beta 2 gap junction protein (Cx26) was expressed in an insect cell line by infection with a baculovirus vector containing the rat beta 2 cDNA. Isolated beta 2 gap junction connexons were reconstituted into planar lipid bilayers. Single channel activity was observed with a unitary conductance of 35-45 pS in 200 mM KCl. Channels with conductance values of 60 pS and 90-110 pS also coexisted with the lower conducting channel suggesting that there are channels with different conductance properties within a population of connexons. Channel activity was observed at voltages of up to 150 mV. Furthermore, the characterization of these channel properties from the beta 2 connexons that were generated by this heterologous expression system has provided the basis for identifying an endogenous beta 2 connexon channel in material reconstituted from native rat liver gap junctions.

Entities: Chemical Disease Gene Species

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Year: 1995 PMID： 7542035 PMCID： PMC1282079 DOI： 10.1016/S0006-3495(95)80353-X

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

45 in total

1. Topological analysis of the major protein in isolated intact rat liver gap junctions and gap junction-derived single membrane structures.

Authors: D B Zimmer; C R Green; W H Evans; N B Gilula
Journal: J Biol Chem Date: 1987-06-05 Impact factor: 5.157

2. Two homologous protein components of hepatic gap junctions.

Authors: B Nicholson; R Dermietzel; D Teplow; O Traub; K Willecke; J P Revel
Journal: Nature Date: 1987 Oct 22-28 Impact factor: 49.962

Review 3. Ion channel subconductance states.

Authors: J A Fox
Journal: J Membr Biol Date: 1987 Impact factor: 1.843

4. Functional assembly of gap junction conductance in lipid bilayers: demonstration that the major 27 kd protein forms the junctional channel.

Authors: J D Young; Z A Cohn; N B Gilula
Journal: Cell Date: 1987-03-13 Impact factor: 41.582

5. Opening of single gap junction channels during formation of electrical coupling between embryonic muscle cells.

Authors: I Chow; S H Young
Journal: Dev Biol Date: 1987-08 Impact factor: 3.582

6. A detergent-independent procedure for the isolation of gap junctions from rat liver.

Authors: E L Hertzberg
Journal: J Biol Chem Date: 1984-08-10 Impact factor: 5.157

7. Voltage gating of connexins.

Authors: T W White; R Bruzzone; D A Goodenough; D L Paul
Journal: Nature Date: 1994-09-15 Impact factor: 49.962

8. Topology of the 32-kd liver gap junction protein determined by site-directed antibody localizations.

Authors: L C Milks; N M Kumar; R Houghten; N Unwin; N B Gilula
Journal: EMBO J Date: 1988-10 Impact factor: 11.598

9. Comparative characterization of the 21-kD and 26-kD gap junction proteins in murine liver and cultured hepatocytes.

Authors: O Traub; J Look; R Dermietzel; F Brümmer; D Hülser; K Willecke
Journal: J Cell Biol Date: 1989-03 Impact factor: 10.539

10. Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA.

Authors: J T Zhang; B J Nicholson
Journal: J Cell Biol Date: 1989-12 Impact factor: 10.539

10 in total

Review 1. Multiple connexin proteins in single intercellular channels: connexin compatibility and functional consequences.

Authors: T W White; R Bruzzone
Journal: J Bioenerg Biomembr Date: 1996-08 Impact factor: 2.945

Single channel behavior of recombinant beta 2 gap junction connexons reconstituted into planar lipid bilayers.

1. Topological analysis of the major protein in isolated intact rat liver gap junctions and gap junction-derived single membrane structures.

2. Two homologous protein components of hepatic gap junctions.

Review 3. Ion channel subconductance states.

4. Functional assembly of gap junction conductance in lipid bilayers: demonstration that the major 27 kd protein forms the junctional channel.

5. Opening of single gap junction channels during formation of electrical coupling between embryonic muscle cells.

6. A detergent-independent procedure for the isolation of gap junctions from rat liver.

7. Voltage gating of connexins.

8. Topology of the 32-kd liver gap junction protein determined by site-directed antibody localizations.

9. Comparative characterization of the 21-kD and 26-kD gap junction proteins in murine liver and cultured hepatocytes.

10. Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA.

Review 1. Multiple connexin proteins in single intercellular channels: connexin compatibility and functional consequences.

Review 2. Electrical coupling and its channels.

3. Heteromeric connexons in lens gap junction channels.

4. The M34A mutant of Connexin26 reveals active conductance states in pore-suspending membranes.

Review 5. Connexin expression systems: to what extent do they reflect the situation in the animal?

6. Structural determinants and proliferative consequences of connexin 37 hemichannel function in insulinoma cells.

7. Projection structure of a N-terminal deletion mutant of connexin 26 channel with decreased central pore density.

8. Physiological role of gap-junctional hemichannels. Extracellular calcium-dependent isosmotic volume regulation.

Review 9. Functional analysis and regulation of purified connexin hemichannels.

10. Connexins/connexons. Cell-free expression.