M Nisolle1, F Casanas-Roux, J Donnez. 1. Department of Gynecology, Catholic University of Louvain, St. Luc Hospital, Brussels, Belgium.
Abstract
OBJECTIVE: To evaluate quantitatively cytokeratin and vimentin staining in glandular and stromal cells of eutopic and ectopic endometrium. DESIGN: The investigation of cytokeratin and vimentin was carried out using the new computerized technology of image analysis. SETTING: University Hospital, Department of Gynecology. PATIENTS: Biopsies were taken from patients undergoing a laparoscopy for infertility (29 endometrial biopsies and 31 biopsies of peritoneal endometriosis). None of them were treated. RESULTS: Cyclic variations of cytokeratin and vimentin staining were noted in eutopic endometrium. The cytokeratin and vimentin staining pattern consistently was lower in ectopic endometrium than in eutopic endometrium. CONCLUSIONS: Endometriotic epithelial and stromal cells undergo a complex program of differentiation giving them histochemical characteristics similar to those observed in endometrium. Such a concomitant expression of antigenicity indicates their close relationship with their mesodermal müllerian origin.
OBJECTIVE: To evaluate quantitatively cytokeratin and vimentin staining in glandular and stromal cells of eutopic and ectopic endometrium. DESIGN: The investigation of cytokeratin and vimentin was carried out using the new computerized technology of image analysis. SETTING: University Hospital, Department of Gynecology. PATIENTS: Biopsies were taken from patients undergoing a laparoscopy for infertility (29 endometrial biopsies and 31 biopsies of peritoneal endometriosis). None of them were treated. RESULTS: Cyclic variations of cytokeratin and vimentin staining were noted in eutopic endometrium. The cytokeratin and vimentin staining pattern consistently was lower in ectopic endometrium than in eutopic endometrium. CONCLUSIONS: Endometriotic epithelial and stromal cells undergo a complex program of differentiation giving them histochemical characteristics similar to those observed in endometrium. Such a concomitant expression of antigenicity indicates their close relationship with their mesodermal müllerian origin.
Authors: Timo Gemoll; Jens K Habermann; Johanna Lahmann; Silke Szymczak; Caroline Lundgren; Nana K Bündgen; Thomas Jungbluth; Britta Nordström; Susanne Becker; Marta I Lomnytska; Hans-Peter Bruch; Andreas Ziegler; Ulf Hellman; Gert Auer; Uwe J Roblick; Hans Jörnvall Journal: Cell Mol Life Sci Date: 2011-07-08 Impact factor: 9.261
Authors: Lisley I Mambelli; Rodrigo C Mattos; Gustavo H Z Winter; Dener S Madeiro; Bruna P Morais; Eduardo Malschitzky; Maria Angélica Miglino; Alexandre Kerkis; Irina Kerkis Journal: PLoS One Date: 2014-06-05 Impact factor: 3.240