T Ushiyama1, K Inoue, J Nishioka. 1. Department of Orthopaedic Surgery, Shiga University of Medical Science, Otsu, Japan.
Abstract
OBJECTIVE: We investigated the expression of estrogen receptors (ER) and the presence of estradiol in human synovial tissues from subjects without arthritis and patients with traumatic arthritis, osteoarthritis, and rheumatoid arthritis. METHODS: Synovial tissues were immunostained using ER-D5 monoclonal antibody (Mab) directed toward an ER related protein (p29), ER-ICA Mab directed toward nuclear ER, and antiestradiol serum. Fluorescent estradiol was used to demonstrate specific estradiol binding to ER. RESULTS: (1) p29 was present predominantly in hyperplastic synovial lining cells and smooth muscles of blood vessels. (2) Exogenous estradiol bound to synoviocytes and sublining macrophage-like cells in the inflamed synovium. (3) Endogenous estradiol was also detected in hyperplastic lining cells and macrophage-like cells in arthritic synovium. CONCLUSION: Our study demonstrated the expression of ER in arthritic synovium and suggested that estrogen modulates local inflammation in various joint diseases via synoviocytes as well as sublining macrophage-like cells.
OBJECTIVE: We investigated the expression of estrogen receptors (ER) and the presence of estradiol in human synovial tissues from subjects without arthritis and patients with traumatic arthritis, osteoarthritis, and rheumatoid arthritis. METHODS: Synovial tissues were immunostained using ER-D5 monoclonal antibody (Mab) directed toward an ER related protein (p29), ER-ICA Mab directed toward nuclear ER, and antiestradiol serum. Fluorescent estradiol was used to demonstrate specific estradiol binding to ER. RESULTS: (1) p29 was present predominantly in hyperplastic synovial lining cells and smooth muscles of blood vessels. (2) Exogenous estradiol bound to synoviocytes and sublining macrophage-like cells in the inflamed synovium. (3) Endogenous estradiol was also detected in hyperplastic lining cells and macrophage-like cells in arthritic synovium. CONCLUSION: Our study demonstrated the expression of ER in arthritic synovium and suggested that estrogen modulates local inflammation in various joint diseases via synoviocytes as well as sublining macrophage-like cells.
Authors: S Guiducci; A Del Rosso; M Cinelli; F Perfetto; R Livi; A Rossi; A Gabrielli; R Giacomelli; N Iori; G Fibbi; M Del Rosso; M Matucci Cerinic Journal: Arthritis Res Ther Date: 2005-09-08 Impact factor: 5.156
Authors: James C Keith; Leo M Albert; Yelena Leathurby; Max Follettie; Lili Wang; Lisa Borges-Marcucci; Christopher C Chadwick; Robert J Steffan; Douglas C Harnish Journal: Arthritis Res Ther Date: 2005-02-21 Impact factor: 5.156