Literature DB >> 7539939

Separation of antigenic glycoprotein fractions from cell-free homogenate of Pseudomonas pseudomallei and characterization as tyrosine phosphatase.

E Kondo1, P Wangroongsaub, P Naigowit, K Kanai.   

Abstract

Cell-free extracts were prepared from Pseudomonas pseudomallei cells by freezing-thawing, sonication, and differential ultracentrifugation. The extracts were subjected to column chromatography with DEAE-sepharose to obtain glycoprotein fractions. The fractions showed acid phosphatase activity to p-nitrophenyl phosphate, tyrosine phosphate, serine phosphate, but not to threonine phosphate. They were highly antigenic when tested by immunofluorescence assay with the sera of melioidosis patients.

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Year:  1994        PMID： 7539939

Source DB:  PubMed          Journal:  Southeast Asian J Trop Med Public Health        ISSN： 0125-1562            Impact factor:   0.267


  3 in total

Review 1.  Environmental factors that affect the survival and persistence of Burkholderia pseudomallei.

Authors:  Timothy J J Inglis; Jose-Luis Sagripanti
Journal:  Appl Environ Microbiol       Date:  2006-09-15       Impact factor: 4.792

2.  Flagellar glycosylation in Burkholderia pseudomallei and Burkholderia thailandensis.

Authors:  Andrew E Scott; Susan M Twine; Kelly M Fulton; Richard W Titball; Angela E Essex-Lopresti; Timothy P Atkins; Joann L Prior
Journal:  J Bacteriol       Date:  2011-05-20       Impact factor: 3.490

3.  Interaction between Burkholderia pseudomallei and Acanthamoeba species results in coiling phagocytosis, endamebic bacterial survival, and escape.

Authors:  T J Inglis; P Rigby; T A Robertson; N S Dutton; M Henderson; B J Chang
Journal:  Infect Immun       Date:  2000-03       Impact factor: 3.441
  3 in total

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