Literature DB >> 7532103

Quantitative and qualitative analysis of exogenous gene expression by the S1 nuclease protection assay.

M E Walmsley1, R K Patient.   

Abstract

A method for determining the beginning and end of gene transcripts along with the position of intron/exon boundaries by S1 nuclease protection is described. Probe strategies and hybridization conditions are considered in detail. Under conditions of probe excess the method is quantitative.

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Year:  1994        PMID： 7532103     DOI： 10.1007/bf02921694

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  6 in total

1.  Mapping of gene transcripts by nuclease protection assays and cDNA primer extension.

Authors:  F J Calzone; R J Britten; E H Davidson
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

2.  Mung bean nuclease I. Physical, chemical, and catalytic properties.

Authors:  D Kowalski; W D Kroeker; M Laskowski
Journal:  Biochemistry       Date:  1976-10-05       Impact factor: 3.162

3.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

4.  Exonuclease VII of Escherichia coli. Mechanism of action.

Authors:  J W Chase; C C Richardson
Journal:  J Biol Chem       Date:  1974-07-25       Impact factor: 5.157

5.  Exonuclease VII of Escherichia coli. Purification and properties.

Authors:  J W Chase; C C Richardson
Journal:  J Biol Chem       Date:  1974-07-25       Impact factor: 5.157

6.  Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.

Authors:  R F Weaver; C Weissmann
Journal:  Nucleic Acids Res       Date:  1979-11-10       Impact factor: 16.971
  6 in total

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