Literature DB >> 7532101

Detection of proteins and sialoglycoproteins in polyacrylamide gels using eosin Y stain.

F Lin1, G E Wise.   

Abstract

An eosin Y staining technique that permits detection of various proteins, including membrane sialoglycoproteins, in polyacrylamide gels is described. The sensitivity of the eosin Y staining method is comparable to silver staining. In addition, there is an added advantage of the antigenicity of the stained proteins being retained in a Western blot. Details of the procedure to obtain optimal staining results are described.

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Year:  1994        PMID： 7532101     DOI： 10.1007/bf02921555

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  10 in total

1.  Transfer of silver-stained proteins from polyacrylamide gels to polyvinylidene difluoride membranes.

Authors:  G E Wise; F Lin
Journal:  J Biochem Biophys Methods       Date:  1991-04

2.  Eosin Y staining of proteins in polyacrylamide gels.

Authors:  F Lin; W Fan; G E Wise
Journal:  Anal Biochem       Date:  1991-08-01       Impact factor: 3.365

3.  Heterogeneity of the sialoglycoproteins of the normal human erythrocyte membrane.

Authors:  T J Mueller; A W Dow; M Morrison
Journal:  Biochem Biophys Res Commun       Date:  1976-09-07       Impact factor: 3.575

4.  Trace polypeptides in cellular extracts and human body fluids detected by two-dimensional electrophoresis and a highly sensitive silver stain.

Authors:  C R Merril; R C Switzer; M L Van Keuren
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels.

Authors:  R C Switzer; C R Merril; S Shifrin
Journal:  Anal Biochem       Date:  1979-09-15       Impact factor: 3.365

6.  Transfer of proteins from acrylamide gels to nitrocellulose paper after silver stain detection.

Authors:  T J Nalty; L C Yeoman
Journal:  J Immunol Methods       Date:  1988-02-24       Impact factor: 2.303

7.  Glycoproteins of cell surfaces. A comparative study of three different cell surfaces of the rat.

Authors:  H Glossmann; D M Neville
Journal:  J Biol Chem       Date:  1971-10-25       Impact factor: 5.157

8.  A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.

Authors:  B R Oakley; D R Kirsch; N R Morris
Journal:  Anal Biochem       Date:  1980-07-01       Impact factor: 3.365

9.  Detection of erythrocyte membrane proteins, sialoglycoproteins, and lipids in the same polyacrylamide gel using a double-staining technique.

Authors:  J K Dzandu; M E Deh; D L Barratt; G E Wise
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

10.  Silver staining of proteins in polyacrylamide gels.

Authors:  W Wray; T Boulikas; V P Wray; R Hancock
Journal:  Anal Biochem       Date:  1981-11-15       Impact factor: 3.365
  10 in total

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