| Literature DB >> 7531985 |
J Marshall1, R Molloy, G W Moss, J R Howe, T E Hughes.
Abstract
Two methods are described for using the jellyfish green fluorescent protein (GFP) as a reporter gene for ion channel expression. GFP fluorescence can be used to identify the transfected cells, and to estimate the relative levels of ion channel expression, in cotransfection experiments. A GFP-NMDAR1 chimera can be constructed that produces a functional, fluorescent receptor subunit. These methods should facilitate studies of ion channel expression, localization, and processing.Entities:
Mesh：
Substances：
Year: 1995 PMID： 7531985 DOI： 10.1016/0896-6273(95)90279-1
Source DB: PubMed Journal: Neuron ISSN： 0896-6273 Impact factor: 17.173