Literature DB >> 7531814

Factors regulating cryIVB expression in the cyanobacterium--Synechococcus PCC 7942.

E Soltes-Rak1, D J Kushner, D D Williams, J R Coleman.   

Abstract

The expression of the larvicidal Bacillus thuringiensis subsp. israelensis cryIVB gene in cyanobacteria has been suggested to be an effective means of controlling mosquito populations. Using a variety of cryIVB constructs, in this study we have examined the effect of Synechococcus PCC 7942 culture age on intracellular toxin levels and have attempted to determine the mechanisms by which cryIVB gene expression is regulated. The data suggest that specific degradation of the cryIVB mRNA limits toxin production; however, the addition of cyanobacterial 3' untranslated DNA sequences to the cryIVB gene did not improve mRNA stability or toxin levels. An analysis of the cryIVB sequence and comparison of codon usage patterns with highly expressed cyanobacterial genes suggest that inefficient translation and intragenic ribosomal binding sites impede protein synthesis and result in rapid turnover of the toxin mRNA.

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Year:  1995        PMID： 7531814     DOI： 10.1007/bf00288602

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  27 in total

1.  Modification of the coding sequence enhances plant expression of insect control protein genes.

Authors:  F J Perlak; R L Fuchs; D A Dean; S L McPherson; D A Fischhoff
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-15       Impact factor: 11.205

2.  Codon replacement in the PGK1 gene of Saccharomyces cerevisiae: experimental approach to study the role of biased codon usage in gene expression.

Authors:  A Hoekema; R A Kastelein; M Vasser; H A de Boer
Journal:  Mol Cell Biol       Date:  1987-08       Impact factor: 4.272

3.  Molecular cloning and sequence analysis of the cyanobacterial gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase.

Authors:  K Shinozaki; C Yamada; N Takahata; M Sugiura
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

4.  Isolation and nucleotide sequence analysis of the ferredoxin I gene from the cyanobacterium Anacystis nidulans R2.

Authors:  M E Reith; D E Laudenbach; N A Straus
Journal:  J Bacteriol       Date:  1986-12       Impact factor: 3.490

5.  Nucleotide sequence of phycocyanin beta-subunit gene of cyanobacterium Anacystis nidulans strain R2.

Authors:  P C Lau; J A Condie; G Alvarado-Urbina; R H Lau
Journal:  Nucleic Acids Res       Date:  1987-03-11       Impact factor: 16.971

6.  Phycocyanin alpha-subunit gene of Anacystis nidulans R2: cloning, nucleotide sequencing and expression in Escherichia coli.

Authors:  R H Lau; G Alvarado-Urbina; P C Lau
Journal:  Gene       Date:  1987       Impact factor: 3.688

7.  Structural similarity between the lepidoptera- and diptera-specific insecticidal endotoxin genes of Bacillus thuringiensis subsp. "kurstaki" and "israelensis".

Authors:  L Thorne; F Garduno; T Thompson; D Decker; M Zounes; M Wild; A M Walfield; T J Pollock
Journal:  J Bacteriol       Date:  1986-06       Impact factor: 3.490

8.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

9.  Selection of cyanobacteria isolated from mosquito breeding sites as a potential food source for mosquito larvae.

Authors:  I Thiery; L Nicolas; R Rippka; N Tandeau de Marsac
Journal:  Appl Environ Microbiol       Date:  1991-05       Impact factor: 4.792

10.  Iron-dependent stability of the ferredoxin I transcripts from the cyanobacterial strains Synechococcus species PCC 7942 and Anabaena species PCC 7937.

Authors:  A Bovy; G de Vrieze; L Lugones; P van Horssen; C van den Berg; M Borrias; P Weisbeek
Journal:  Mol Microbiol       Date:  1993-02       Impact factor: 3.501
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  6 in total

1.  Toward mosquito control with a green alga: Expression of Cry toxins of Bacillus thuringiensis subsp. israelensis (Bti) in the chloroplast of Chlamydomonas.

Authors:  Seongjoon Kang; Obed W Odom; Saravanan Thangamani; David L Herrin
Journal:  J Appl Phycol       Date:  2016-11-23       Impact factor: 3.215

2.  Expression of mouse metallothionein in the cyanobacterium Synechococcus PCC7942.

Authors:  J L Erbe; K B Taylor; L M Hall
Journal:  J Ind Microbiol       Date:  1996-07

3.  Mosquito larvicidal activity of transgenic Anabaena strain PCC 7120 expressing combinations of genes from Bacillus thuringiensis subsp. israelensis.

Authors:  W Xiaoqiang; S J Vennison; L Huirong; E Ben-Dov; A Zaritsky; S Boussiba
Journal:  Appl Environ Microbiol       Date:  1997-12       Impact factor: 4.792

4.  Identification and characterization of a gene encoding a vertebrate-type carbonic anhydrase in cyanobacteria.

Authors:  E Soltes-Rak; M E Mulligan; J R Coleman
Journal:  J Bacteriol       Date:  1997-02       Impact factor: 3.490

Review 5.  Cyanobacterial toxins as allelochemicals with potential applications as algaecides, herbicides and insecticides.

Authors:  John P Berry; Miroslav Gantar; Mario H Perez; Gerald Berry; Fernando G Noriega
Journal:  Mar Drugs       Date:  2008-05-15       Impact factor: 5.118

6.  Expression of a Synthetic Gene for the Major Cytotoxin (Cyt1Aa) of Bacillus thuringiensis subsp. israelensis in the Chloroplast of Wild-Type Chlamydomonas.

Authors:  Seongjoon Kang; Obed W Odom; Candice L Malone; Saravanan Thangamani; David L Herrin
Journal:  Biology (Basel)       Date:  2018-05-08
  6 in total

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