OBJECTIVE: To determine if DNA methylation inhibitors make T cells autoreactive by inducing lymphocyte function-associated antigen type 1 (LFA-1) (CD11a/CD18) overexpression. METHODS: T cell clones were treated with 3 distinct DNA methylation inhibitors or were stably transfected with a CD18 cDNA in a mammalian expression vector, and the effects on LFA-1 expression and activation requirements were examined. RESULTS: LFA-1 overexpression, caused by DNA methylation inhibitors or by transfection, correlates with the development of autoreactivity. CONCLUSION: LFA-1 overexpression may contribute to T cell autoreactivity.
OBJECTIVE: To determine if DNA methylation inhibitors make T cells autoreactive by inducing lymphocyte function-associated antigen type 1 (LFA-1) (CD11a/CD18) overexpression. METHODS: T cell clones were treated with 3 distinct DNA methylation inhibitors or were stably transfected with a CD18 cDNA in a mammalian expression vector, and the effects on LFA-1 expression and activation requirements were examined. RESULTS:LFA-1 overexpression, caused by DNA methylation inhibitors or by transfection, correlates with the development of autoreactivity. CONCLUSION:LFA-1 overexpression may contribute to T cell autoreactivity.
Authors: R Yung; D Powers; K Johnson; E Amento; D Carr; T Laing; J Yang; S Chang; N Hemati; B Richardson Journal: J Clin Invest Date: 1996-06-15 Impact factor: 14.808
Authors: A H Sawalha; M Jeffries; R Webb; Q Lu; G Gorelik; D Ray; J Osban; N Knowlton; K Johnson; B Richardson Journal: Genes Immun Date: 2008-06 Impact factor: 2.676