Literature DB >> 7524440

Identifying members of the domain Archaea with rRNA-targeted oligonucleotide probes.

S Burggraf1, T Mayer, R Amann, S Schadhauser, C R Woese, K O Stetter.   

Abstract

Two 16S rRNA-targeted oligonucleotide probes were designed for the archaeal kingdoms Euryachaeota and Crenarchaeota. Probe specificities were evaluated by nonradioactive dot blot hybridization against selected reference organisms. The successful application of fluorescent-probe derivatives for whole-cell hybridization required organism-specific optimizations of fixation and hybridization conditions to assure probe penetration and morphological integrity of the cells. The probes allowed preliminary grouping of three new hyperthermophilic isolates. Together with other group-specific rRNA-targeted oligonucleotide probes, these probes will facilitate rapid in situ monitoring of the populations present in hydrothermal systems and support cultivation attempts.

Keywords:  Non-programmatic

Mesh：

Substances：

Year:  1994        PMID： 7524440      PMCID： PMC201778          DOI： 10.1128/aem.60.9.3112-3119.1994

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  23 in total

1.  Methanopyrus kandleri: an archaeal methanogen unrelated to all other known methanogens.

Authors:  S Burggraf; K O Stetter; P Rouviere; C R Woese
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2.  Archaea in coastal marine environments.

Authors:  E F DeLong
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-15       Impact factor: 11.205

3.  Isolation of extremely thermophilic sulfate reducers: evidence for a novel branch of archaebacteria.

Authors:  K O Stetter; G Lauerer; M Thomm; A Neuner
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4.  Studies on the methane fermentation. X. A new formate-decomposing bacterium, Methanococcus vannielii.

Authors:  T C STADTMAN; H A BARKER
Journal:  J Bacteriol       Date:  1951-09       Impact factor: 3.490

Review 5.  Bacterial evolution.

Authors:  C R Woese
Journal:  Microbiol Rev       Date:  1987-06

6.  Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells.

Authors:  E F DeLong; G S Wickham; N R Pace
Journal:  Science       Date:  1989-03-10       Impact factor: 47.728

7.  Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.

Authors:  J Brosius; M L Palmer; P J Kennedy; H F Noller
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

8.  A comparison of the 16S ribosomal RNAs from mesophilic and thermophilic bacilli: some modifications in the Sanger method for RNA sequencing.

Authors:  C Woese; M Sogin; D Stahl; B J Lewis; L Bonen
Journal:  J Mol Evol       Date:  1976-04-09       Impact factor: 2.395

9.  Pyrobaculum aerophilum sp. nov., a novel nitrate-reducing hyperthermophilic archaeum.

Authors:  P Völkl; R Huber; E Drobner; R Rachel; S Burggraf; A Trincone; K O Stetter
Journal:  Appl Environ Microbiol       Date:  1993-09       Impact factor: 4.792

10.  Probing activated sludge with oligonucleotides specific for proteobacteria: inadequacy of culture-dependent methods for describing microbial community structure.

Authors:  M Wagner; R Amann; H Lemmer; K H Schleifer
Journal:  Appl Environ Microbiol       Date:  1993-05       Impact factor: 4.792
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  37 in total

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Authors:  K Takai; K Horikoshi
Journal:  Genetics       Date:  1999-08       Impact factor: 4.562

Review 2.  Bioenergetics of the Archaea.

Authors:  G Schäfer; M Engelhard; V Müller
Journal:  Microbiol Mol Biol Rev       Date:  1999-09       Impact factor: 11.056

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Journal:  Appl Environ Microbiol       Date:  2001-05       Impact factor: 4.792

4.  Molecular characterization of community structures and sulfur metabolism within microbial streamers in Japanese hot springs.

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5.  Archaeal nucleic acids in picoplankton from great lakes on three continents.

Authors:  B P Keough; T M Schmidt; R E Hicks
Journal:  Microb Ecol       Date:  2003-08       Impact factor: 4.552

6.  FISH shows that Desulfotomaculum spp. are the dominating sulfate-reducing bacteria in a pristine aquifer.

Authors:  J Detmers; H Strauss; U Schulte; A Bergmann; K Knittel; J Kuever
Journal:  Microb Ecol       Date:  2003-10-23       Impact factor: 4.552

7.  Anaerobic oxidation of methane at different temperature regimes in Guaymas Basin hydrothermal sediments.

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Journal:  ISME J       Date:  2011-11-17       Impact factor: 10.302

8.  Making all parts of the 16S rRNA of Escherichia coli accessible in situ to single DNA oligonucleotides.

Authors:  L Safak Yilmaz; Hatice E Okten; Daniel R Noguera
Journal:  Appl Environ Microbiol       Date:  2006-01       Impact factor: 4.792

9.  Fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes to identify small phytoplankton by flow cytometry.

Authors:  N Simon; N LeBot; D Marie; F Partensky; D Vaulot
Journal:  Appl Environ Microbiol       Date:  1995-07       Impact factor: 4.792

10.  Membrane lipid patterns typify distinct anaerobic methanotrophic consortia.

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Journal:  Proc Natl Acad Sci U S A       Date:  2004-07-16       Impact factor: 11.205
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