OBJECTIVES: To assess the efficacy of oral fluid antibody testing for the detection of hepatitis C. METHODS: Paired serum and oral fluid collections were obtained from 216 subjects. A modification of the serum HCV ELISA assay was developed to improve test accuracy for an oral fluid substrate. Sensitivity was determined in 109 HCV serum ELISA-positive patients and specificity in 107 HCV serum ELISA-negative patients. RESULTS: Overall sensitivity of oral fluid collection and testing was 98.2%; specificity was 99.1%. These parameters did not seem to be altered by presence of concurrent hepatitis B infection, inflammatory state of the liver, or other factors. CONCLUSIONS: Oral fluid collection and HCV antibody testing by the modified ELISA method seems to be an effective and efficient alternatives to venipuncture and serum HCV antibody testing. Their use may facilitate epidemiological surveys and evaluation of individual patients when blood collection is not feasible.
OBJECTIVES: To assess the efficacy of oral fluid antibody testing for the detection of hepatitis C. METHODS: Paired serum and oral fluid collections were obtained from 216 subjects. A modification of the serum HCV ELISA assay was developed to improve test accuracy for an oral fluid substrate. Sensitivity was determined in 109 HCV serum ELISA-positive patients and specificity in 107 HCV serum ELISA-negative patients. RESULTS: Overall sensitivity of oral fluid collection and testing was 98.2%; specificity was 99.1%. These parameters did not seem to be altered by presence of concurrent hepatitis B infection, inflammatory state of the liver, or other factors. CONCLUSIONS: Oral fluid collection and HCV antibody testing by the modified ELISA method seems to be an effective and efficient alternatives to venipuncture and serum HCV antibody testing. Their use may facilitate epidemiological surveys and evaluation of individual patients when blood collection is not feasible.
Authors: Letícia de Paula Scalioni; Helena Medina Cruz; Vanessa Salete de Paula; Jaqueline Corrêia Oliveira; Renata Tourinho Dos Santos; Ana Rita Coimbra Motta-Castro; Paula Guerra Murat; Cristiane Alves Villela-Nogueira; Lia Laura Lewis-Ximenez; Elisabeth Lampe; Livia Melo Villar Journal: J Clin Lab Anal Date: 2013-02-25 Impact factor: 2.352
Authors: V González; E Martró; C Folch; A Esteve; L Matas; A Montoliu; J R Grífols; F Bolao; C Tural; R Muga; J V Parry; V Ausina; J Casabona Journal: Eur J Clin Microbiol Infect Dis Date: 2007-11-20 Impact factor: 3.267
Authors: Prithivi Chellamuthu; Aaron N Angel; Melanie A MacMullan; Nicholas Denny; Aubree Mades; Marilisa Santacruz; Ronell Lopez; Cedie Bagos; Joseph G Casian; Kylie Trettner; Lauren Lopez; Nina Nirema; Matthew Brobeck; Noah Kojima; Jeffrey D Klausner; Fred Turner; Vladimir Slepnev; Albina Ibrayeva Journal: Front Immunol Date: 2021-12-09 Impact factor: 7.561