| Literature DB >> 7523537 |
M E Helminen1, I Maciver, J L Latimer, J Klesney-Tait, L D Cope, M Paris, G H McCracken, E J Hansen.
Abstract
A monoclonal antibody (MAb) to Moraxella catarrhalis O35E bound to a surface-exposed epitope of a proteinaceous antigen of this organism. The antigen, designated UspA, was present in every strain of the pathogen tested in a colony blot RIA. UspA had a molecular mass on SDS-PAGE that varied between 300 and 400 kDa, depending on the individual M. catarrhalis strain. Passive immunization of mice with the UspA-reactive Mab enhanced pulmonary clearance of M. catarrhalis. Use of this Mab to screen a M. catarrhalis genomic DNA library permitted identification of a recombinant bacteriophage expressing the M. catarrhalis UspA protein. The recombinant UspA protein was used in Western blot analysis with sera from patients with M. catarrhalis pneumonia. Convalescent-phase sera but not acute-phase sera from these patients contained antibodies to this M. catarrhalis surface protein, indicating that M. catarrhalis strains growing in vivo express this molecule.Entities:
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Year: 1994 PMID： 7523537 DOI： 10.1093/infdis/170.4.867
Source DB: PubMed Journal: J Infect Dis ISSN： 0022-1899 Impact factor: 5.226