Literature DB >> 7522589

A rapid immunostaining method for frozen sections.

M Chilosi1, M Lestani, S Pedron, L Montagna, A Benedetti, G Pizzolo, F Menestrina.   

Abstract

A simple and rapid one-step method for demonstrating immunohistochemical markers (leukocyte common antigen, cytokeratin, etc.) is described, which can help define the nature of poorly differentiated neoplasms for diagnosis using frozen section. Microwave irradiation was used to speed immunohistochemical analysis using "Enhanced Polymer One-step Staining" (EPOS) reagents on cryostat sections from a variety of pathologic samples. Reproducible results were obtained using EPOS reagents for leukocyte common antigen and cytokeratin. The overall procedure takes less than 10 min and can be completed during surgery.

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Year:  1994        PMID： 7522589     DOI： 10.3109/10520299409106292

Source DB:  PubMed          Journal:  Biotech Histochem        ISSN： 1052-0295            Impact factor:   1.718


  6 in total

Review 1.  Sentinel node detection in pre-operative axillary staging.

Authors:  Giuseppe Trifirò; Giuseppe Viale; Oreste Gentilini; Laura Lavinia Travaini; Giovanni Paganelli
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Review 2.  Pathologic examination of the axillary sentinel lymph nodes in patients with early-stage breast carcinoma: current and resolving controversies on the basis of the European Institute of Oncology experience.

Authors:  Giuseppe Viale; Mauro G Mastropasqua; Eugenio Maiorano; Giovanni Mazzarol
Journal:  Virchows Arch       Date:  2005-11-24       Impact factor: 4.064

3.  Protocol for ultrarapid immunostaining of frozen sections.

Authors:  T Richter; J Nährig; P Komminoth; J Kowolik; M Werner
Journal:  J Clin Pathol       Date:  1999-06       Impact factor: 3.411

4.  Intraoperative imprint cytologic evaluation of sentinel lymph nodes for lobular carcinoma of the breast.

Authors:  Andrew J Creager; Kim R Geisinger; Nancy D Perrier; Perry Shen; Jo Ann Shaw; Peter R Young; Doug Case; Edward A Levine
Journal:  Ann Surg       Date:  2004-01       Impact factor: 12.969

5.  The EnVision++ system: a new immunohistochemical method for diagnostics and research. Critical comparison with the APAAP, ChemMate, CSA, LABC, and SABC techniques.

Authors:  E Sabattini; K Bisgaard; S Ascani; S Poggi; M Piccioli; C Ceccarelli; F Pieri; G Fraternali-Orcioni; S A Pileri
Journal:  J Clin Pathol       Date:  1998-07       Impact factor: 3.411

Review 6.  Quantitative measurement of cancer tissue biomarkers in the lab and in the clinic.

Authors:  Daniel E Carvajal-Hausdorf; Kurt A Schalper; Veronique M Neumeister; David L Rimm
Journal:  Lab Invest       Date:  2014-12-15       Impact factor: 5.662
  6 in total

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