Literature DB >> 7521850

Fluorescent differential display: arbitrarily primed RT-PCR fingerprinting on an automated DNA sequencer.

T Ito1, K Kito, N Adati, Y Mitsui, H Hagiwara, Y Sakaki.   

Abstract

We established robust, reliable protocols for 'Differential Display (DD),' an RNA fingerprinting method originally developed by Liang and Pardee [(1992) Science 257, 967-971] using RT-PCR with arbitrary primers. Our protocols are optimized so that reliable DD analysis can be performed on a fluorescent DNA sequencer to ensure high throughput as well as improved operational safety, compared with the original one using radioactive compounds. Such 'Fluorescent Differential Display (FDD)' techniques will accelerate the identification of differentially expressed as well as polymorphic transcripts to address various biological questions.

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Year:  1994        PMID： 7521850     DOI： 10.1016/0014-5793(94)00867-1

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  28 in total

1.  Dual transforming activities of the FUS (TLS)-ERG leukemia fusion protein conferred by two N-terminal domains of FUS (TLS).

Authors:  H Ichikawa; K Shimizu; R Katsu; M Ohki
Journal:  Mol Cell Biol       Date:  1999-11       Impact factor: 4.272

2.  Soldier caste-specific gene expression in the mandibular glands of Hodotermopsis japonica (Isoptera: termopsidae).

Authors:  T Miura; A Kamikouchi; M Sawata; H Takeuchi; S Natori; T Kubo; T Matsumoto
Journal:  Proc Natl Acad Sci U S A       Date:  1999-11-23       Impact factor: 11.205

3.  Differential expression of RAB5A in human lung adenocarcinoma cells with different metastasis potential.

Authors:  L Yu; F Hui-chen; Y Chen; R Zou; S Yan; L Chun-xiang; W Wu-ru; P Li
Journal:  Clin Exp Metastasis       Date:  1999-05       Impact factor: 5.150

4.  Multiplex polymerase chain reaction (PCR) with color-tagged module-shuffling primers for comparing gene expression levels in various cells.

Authors:  C Uematsu; J Nishida; K Okano; F Miura; T Ito; Y Sakaki; H Kambara
Journal:  Nucleic Acids Res       Date:  2001-08-15       Impact factor: 16.971

5.  Induction of c-met proto-oncogene expression at the metastatic site.

Authors:  J Imai; M Watanabe; M Sasaki; R Yamaguchi; S Tateyama; S Sugano
Journal:  Clin Exp Metastasis       Date:  1999-07       Impact factor: 5.150

6.  OsARF1, an auxin response factor from rice, is auxin-regulated and classifies as a primary auxin responsive gene.

Authors:  Frank Waller; Masaki Furuya; Peter Nick
Journal:  Plant Mol Biol       Date:  2002-10       Impact factor: 4.076

7.  Identification of a reproductive-specific, putative lipid transport protein gene in a queenless ponerine ant Diacamma sp.

Authors:  Yasukazu Okada; Satoshi Miyazaki; Shigeyuki Koshikawa; Richard Cornette; Kiyoto Maekawa; Kazuki Tsuji; Toru Miura
Journal:  Naturwissenschaften       Date:  2010-09-25

8.  Screening for imprinted genes by allelic message display: identification of a paternally expressed gene impact on mouse chromosome 18.

Authors:  Y Hagiwara; M Hirai; K Nishiyama; I Kanazawa; T Ueda; Y Sakaki; T Ito
Journal:  Proc Natl Acad Sci U S A       Date:  1997-08-19       Impact factor: 11.205

9.  Differential display analysis reveals the expression of glutathione S-transferase omega and novel genes through an ITAM-containing receptor in ascidian immunocytes.

Authors:  Kaoru Azumi; Takeshi Sasaki; Kazuki Okochi; Sho Yamasaki; Takashi Saito; Hajime Takayama; Hideyoshi Yokosawa
Journal:  Immunogenetics       Date:  2005-07-07       Impact factor: 2.846

10.  Recovering and reamplifying of the differentially expressed cDNA bands isolated from mRNA differential display. A modified method.

Authors:  H Wang; Z Xia; Y Wang; C Li; L Y Sun
Journal:  Mol Biotechnol       Date:  1998-04       Impact factor: 2.695
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