Literature DB >> 7521550

A rapid method for the analysis of influenza virus genes: application to the reassortment of equine influenza virus genes.

C A Adeyefa1, K Quayle, J W McCauley.   

Abstract

We describe a rapid method for genetic characterisation of influenza virus genes using reverse transcription and amplification by polymerase chain reaction (RT/PCR) of all virus segments simultaneously (multiplex RT/PCR) using primers based on the conserved terminal sequences. The product has been shown to be suitable for determination of partial nucleotide sequences which can be used to search nucleotide sequence databases and rapidly map the genetic origin of each segment. We illustrate the use of the method by analysing genetic reassortment in H7N7 equine influenza viruses.

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Year:  1994        PMID： 7521550     DOI： 10.1016/0168-1702(94)90087-6

Source DB:  PubMed          Journal:  Virus Res        ISSN： 0168-1702            Impact factor:   3.303


  10 in total

1.  A simple restriction fragment length polymorphism-based strategy that can distinguish the internal genes of human H1N1, H3N2, and H5N1 influenza A viruses.

Authors:  L A Cooper; K Subbarao
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

2.  Antigenic and genetic analysis of equine influenza viruses from tropical Africa in 1991.

Authors:  C A Adeyefa; M L James; J W McCauley
Journal:  Epidemiol Infect       Date:  1996-10       Impact factor: 2.451

3.  Mutational changes in the hemagglutinin of equine H3 influenza viruses result in the introduction of a glycosylation site which enhances the infectivity of the viruses.

Authors:  C A Adeyefa; J W McCauley; O Tomori
Journal:  Folia Microbiol (Praha)       Date:  1997       Impact factor: 2.099

4.  Use of semiconductor-based oligonucleotide microarrays for influenza a virus subtype identification and sequencing.

Authors:  Michael J Lodes; Dominic Suciu; Mark Elliott; Axel G Stover; Marty Ross; Marcelo Caraballo; Kim Dix; James Crye; Richard J Webby; Wanda J Lyon; David L Danley; Andrew McShea
Journal:  J Clin Microbiol       Date:  2006-04       Impact factor: 5.948

5.  Genome-scale evolution and phylodynamics of equine H3N8 influenza A virus.

Authors:  Pablo R Murcia; James L N Wood; Edward C Holmes
Journal:  J Virol       Date:  2011-03-23       Impact factor: 5.103

6.  Single-reaction genomic amplification accelerates sequencing and vaccine production for classical and Swine origin human influenza a viruses.

Authors:  Bin Zhou; Matthew E Donnelly; Derek T Scholes; Kirsten St George; Masato Hatta; Yoshihiro Kawaoka; David E Wentworth
Journal:  J Virol       Date:  2009-07-15       Impact factor: 5.103

7.  Molecular detection and identification of influenza viruses by oligonucleotide microarray hybridization.

Authors:  Srikumar Sengupta; Kenji Onodera; Alexander Lai; Ulrich Melcher
Journal:  J Clin Microbiol       Date:  2003-10       Impact factor: 5.948

8.  An efficient genome sequencing method for equine influenza [H3N8] virus reveals a new polymorphism in the PA-X protein.

Authors:  Adam Rash; Alana Woodward; Neil Bryant; John McCauley; Debra Elton
Journal:  Virol J       Date:  2014-09-02       Impact factor: 4.099

9.  Analysis of the genetic diversity of influenza A viruses using next-generation DNA sequencing.

Authors:  Silvie Van den Hoecke; Judith Verhelst; Marnik Vuylsteke; Xavier Saelens
Journal:  BMC Genomics       Date:  2015-02-14       Impact factor: 3.969

10.  Comprehensive detection and identification of seven animal coronaviruses and human respiratory coronavirus 229E with a microarray hybridization assay.

Authors:  Qin Chen; Jian Li; Zhirui Deng; Wei Xiong; Quan Wang; Yong-Qiang Hu
Journal:  Intervirology       Date:  2009-12-03       Impact factor: 1.763
  10 in total

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