Literature DB >> 7521000

Efficient synthesis of 32P-labeled single-stranded DNA probes using linear PCR; application of the method for analysis of strand-specific DNA repair.

H J Ruven1, C M Seelen, P H Lohman, L H Mullenders, A A van Zeeland.   

Abstract

We have developed a rapid method to synthesize radioactively labeled single-stranded DNA probes suitable for strand-specific analysis of single copy genes on Southern blot. Linear PCR with 10 microCi alpha 32P-dATP (3000 Ci/mmol) as the only dATP source enabled us to generate strand-specific DNA probes with high specific activity. The probes synthesized by this method have higher specific activities and the same strand specificity compared to the end-labeled single-stranded DNA probes obtained from single-stranded M13mp18/19 vectors. Application of the method for strand-specific analysis of ultraviolet-induced DNA lesions in defined DNA sequences significantly improved the hybridization signal.

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Year:  1994        PMID： 7521000     DOI： 10.1016/0921-8777(94)90018-3

Source DB:  PubMed          Journal:  Mutat Res        ISSN： 0027-5107            Impact factor:   2.433


  9 in total

1.  Nucleotide excision repair in rat male germ cells: low level of repair in intact cells contrasts with high dual incision activity in vitro.

Authors:  J Jansen; A K Olsen; R Wiger; H Naegeli; P de Boer; F van Der Hoeven; J A Holme; G Brunborg; L Mullenders
Journal:  Nucleic Acids Res       Date:  2001-04-15       Impact factor: 16.971

2.  Architecture of the replication fork stalled at the 3' end of yeast ribosomal genes.

Authors:  M Gruber; R E Wellinger; J M Sogo
Journal:  Mol Cell Biol       Date:  2000-08       Impact factor: 4.272

3.  The sensitivity of Cockayne's syndrome cells to DNA-damaging agents is not due to defective transcription-coupled repair of active genes.

Authors:  M F van Oosterwijk; A Versteeg; R Filon; A A van Zeeland; L H Mullenders
Journal:  Mol Cell Biol       Date:  1996-08       Impact factor: 4.272

4.  The sensitivity of human fibroblasts to N-acetoxy-2-acetylaminofluorene is determined by the extent of transcription-coupled repair, and/or their capability to counteract RNA synthesis inhibition.

Authors:  M F van Oosterwijk; R Filon; W H Kalle; L H Mullenders; A A van Zeeland
Journal:  Nucleic Acids Res       Date:  1996-12-01       Impact factor: 16.971

5.  RNase-sensitive DNA modification(s) initiates S. pombe mating-type switching.

Authors:  Sonya Vengrova; Jacob Z Dalgaard
Journal:  Genes Dev       Date:  2004-04-01       Impact factor: 11.361

6.  Monitoring Recombination During Meiosis in Budding Yeast.

Authors:  Shannon Owens; Shangming Tang; Neil Hunter
Journal:  Methods Enzymol       Date:  2018-02-01       Impact factor: 1.600

7.  Identification of a novel type of spacer element required for imprinting in fission yeast.

Authors:  Suha Sayrac; Sonya Vengrova; Emma L Godfrey; Jacob Z Dalgaard
Journal:  PLoS Genet       Date:  2011-03-10       Impact factor: 5.917

8.  Transcription-coupled repair removes both cyclobutane pyrimidine dimers and 6-4 photoproducts with equal efficiency and in a sequential way from transcribed DNA in xeroderma pigmentosum group C fibroblasts.

Authors:  A van Hoffen; J Venema; R Meschini; A A van Zeeland; L H Mullenders
Journal:  EMBO J       Date:  1995-01-16       Impact factor: 11.598

9.  The 3' processing factor CstF functions in the DNA repair response.

Authors:  Nurit Mirkin; Danae Fonseca; Samia Mohammed; Murat A Cevher; James L Manley; Frida E Kleiman
Journal:  Nucleic Acids Res       Date:  2008-02-05       Impact factor: 16.971
  9 in total

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