Literature DB >> 75210

Immunofluorescent anti-tubulin staining of spindles during meiotic maturation of mouse oocytes in vitro.

P M Wassarman, K Fujiwara.   

Abstract

Immunofluorescent anti-tubulin staining has been used to follow nuclear progression from dictyate to metaphase II during meiotic maturation of mouse oocytes in vitro. Antibody directed against tubulin isolated from sea-urchin eggs decorates the metaphase I and metaphase II spindles, as well as the cytoplasmic bridge, midbody, and polar body of the maturing mouse oocytes. Changes in the tubulin-specific staining pattern during meiotic maturation in vitro take place in a highly reproducible manner. Oocytes exposed continuously to cytochalasin B arrest at metaphase I and display a spindle which by immunofluorescent staining is virtually indistinguishable from the spindle of untreated oocytes.

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Year:  1978        PMID： 75210     DOI： 10.1242/jcs.29.1.171

Source DB:  PubMed          Journal:  J Cell Sci        ISSN： 0021-9533            Impact factor:   5.285


  9 in total

1.  Microtubule mediation of cytoplasmic and nuclear maturation during the early stages of resumed meiosis in cultured mouse oocytes.

Authors:  J Van Blerkom
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-01       Impact factor: 11.205

2.  The CBA mouse as a model for age-related aneuploidy in man: studies of oocyte maturation, spindle formation and chromosome alignment during meiosis.

Authors:  U Eichenlaub-Ritter; A C Chandley; R G Gosden
Journal:  Chromosoma       Date:  1988       Impact factor: 4.316

3.  Effects of cold shock on microtubule organization and cell cycle in gynogenetically activated eggs of olive flounder (Paralichthys olivaceus).

Authors:  Xiang-Ping Zhu; Feng You; Pei-Jun Zhang; Yong-Li Xu; Jian-He Xu
Journal:  Mar Biotechnol (NY)       Date:  2006-02-28       Impact factor: 3.619

4.  Microtubule configurations during fertilization, mitosis, and early development in the mouse and the requirement for egg microtubule-mediated motility during mammalian fertilization.

Authors:  G Schatten; C Simerly; H Schatten
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

5.  Slow and ultrarapid freezing of fully grown germinal vesicle-stage mouse oocytes: optimization of survival rate outweighed by defective blastocyst formation.

Authors:  J C Van der Elst; S S Nerinckx; A C Van Steirteghem
Journal:  J Assist Reprod Genet       Date:  1993-04       Impact factor: 3.412

6.  Alterations to the microtubular cytoskeleton and increased disorder of chromosome alignment in spontaneously ovulated mouse oocytes aged in vivo: an immunofluorescence study.

Authors:  U Eichenlaub-Ritter; A C Chandley; R G Gosden
Journal:  Chromosoma       Date:  1986       Impact factor: 4.316

7.  Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation.

Authors:  Santanu De; Douglas Kline
Journal:  BMC Dev Biol       Date:  2013-04-01       Impact factor: 1.978

8.  Non-spindle microtubule organizing centers in metaphase II-arrested mouse oocytes.

Authors:  B Maro; S K Howlett; M Webb
Journal:  J Cell Biol       Date:  1985-11       Impact factor: 10.539

9.  Distribution of tubulin-containing structures in the egg of the sea urchin Strongylocentrotus purpuratus from fertilization through first cleavage.

Authors:  P Harris; M Osborn; K Weber
Journal:  J Cell Biol       Date:  1980-03       Impact factor: 10.539
  9 in total

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