Literature DB >> 7519835

A general purification procedure for chemically synthesized oligoribonucleotides.

J B Murray1, A K Collier, J R Arnold.   

Abstract

A general procedure for the purification of chemically synthesized oligoribonucleotides is reported. Purification based on the use of a single reverse-phase HPLC column with buffer systems of differing ion-pairing capacity is described. These methods have been applied to the preparation of a series of RNAs which range in size from 10 to 46 nucleotides. The yields obtained are high, up to 53% (based on isolated product compared to those obtained from final trityl assay). The purity of the isolated material is 96-99%. Thus with this general procedure, milligram quantities of extremely pure RNA can be efficiently obtained.

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Year:  1994        PMID： 7519835     DOI： 10.1006/abio.1994.1157

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  23 in total

1.  Structural basis of pyrimidine specificity in the MS2 RNA hairpin-coat-protein complex.

Authors:  E Grahn; T Moss; C Helgstrand; K Fridborg; M Sundaram; K Tars; H Lago; N J Stonehouse; D R Davis; P G Stockley; L Liljas
Journal:  RNA       Date:  2001-11       Impact factor: 4.942

2.  Deletion of a single hydrogen bonding atom from the MS2 RNA operator leads to dramatic rearrangements at the RNA-coat protein interface.

Authors:  E Grahn; N J Stonehouse; C J Adams; K Fridborg; L Beigelman; J Matulic-Adamic; S L Warriner; P G Stockley; L Liljas
Journal:  Nucleic Acids Res       Date:  2000-12-01       Impact factor: 16.971

3.  Investigating the structural basis of purine specificity in the structures of MS2 coat protein RNA translational operator hairpins.

Authors:  Charlotte Helgstrand; Elin Grahn; Timothy Moss; Nicola J Stonehouse; Kaspars Tars; Peter G Stockley; Lars Liljas
Journal:  Nucleic Acids Res       Date:  2002-06-15       Impact factor: 16.971

4.  Probing sequence-specific RNA recognition by the bacteriophage MS2 coat protein.

Authors:  P G Stockley; N J Stonehouse; J B Murray; S T Goodman; S J Talbot; C J Adams; L Liljas; K Valegård
Journal:  Nucleic Acids Res       Date:  1995-07-11       Impact factor: 16.971

5.  Crystallographic studies of RNA hairpins in complexes with recombinant MS2 capsids: implications for binding requirements.

Authors:  E Grahn; N J Stonehouse; J B Murray; S van den Worm; K Valegård; K Fridborg; P G Stockley; L Liljas
Journal:  RNA       Date:  1999-01       Impact factor: 4.942

6.  Dissecting the key recognition features of the MS2 bacteriophage translational repression complex.

Authors:  H Lago; S A Fonseca; J B Murray; N J Stonehouse; P G Stockley
Journal:  Nucleic Acids Res       Date:  1998-03-01       Impact factor: 16.971

7.  Antibiotic interactions with the hammerhead ribozyme:tetracyclines as a new class of hammerhead inhibitor.

Authors:  J B Murray; J R Arnold
Journal:  Biochem J       Date:  1996-08-01       Impact factor: 3.857

8.  Crystal structures of MS2 coat protein mutants in complex with wild-type RNA operator fragments.

Authors:  S H van den Worm; N J Stonehouse; K Valegârd; J B Murray; C Walton; K Fridborg; P G Stockley; L Liljas
Journal:  Nucleic Acids Res       Date:  1998-03-01       Impact factor: 16.971

9.  Selection and characterization of RNA aptamers to the RNA-dependent RNA polymerase from foot-and-mouth disease virus.

Authors:  Mark Ellingham; David H J Bunka; David J Rowlands; Nicola J Stonehouse
Journal:  RNA       Date:  2006-10-03       Impact factor: 4.942

10.  Characterization of RNA aptamers that disrupt the RUNX1-CBFbeta/DNA complex.

Authors:  Jenny L Barton; David H J Bunka; Stuart E Knowling; Pascal Lefevre; Alan J Warren; Constanze Bonifer; Peter G Stockley
Journal:  Nucleic Acids Res       Date:  2009-09-09       Impact factor: 16.971
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