PhoE protein as a carrier for foreign epitopes.

Outer membrane protein PhoE of E. coli appears to be a suitable carrier for the expression of foreign antigenic determinants at the bacterial cell-surface. Insertion of stretches of amino acids in the cell-surface exposed regions of PhoE does not interfere with the biogenesis of the protein. Dependent on the cell-surface exposed loop used for insertion and the character of the inserted amino acids up to 50 amino acids could be inserted. Both B-cell epitopes and T-cell epitopes remain antigenic and immunogenic in the PhoE-associated conformation. However, flanking amino acids can interfere with the antigenicity and immunogenicity of T-cell epitopes inserted in PhoE. Because E. coli PhoE can be expressed in attenuated Salmonella and Shigella strains, it seems to be a suitable vaccine carrier candidate.