AIMS: To evaluate a safe sputum processing method for detection of tuberculosis in developing countries. METHODS: A sample processing method was developed in which acid fast bacilli were killed with 1% sodium hypochlorite and concentrated by flotation on a layer of xylene before staining by the Ziehl Neelsen or auramine O methods. RESULTS: Best results were obtained by auramine O staining after flotation. Staining by the Ziehl Neelsen method after flotation gave better results than direct Ziehl Neelsen staining without flotation. CONCLUSIONS: The flotation method with Ziehl Neelsen staining offers advantages for smear preparation in the tuberculosis control programmes of developing countries.
AIMS: To evaluate a safe sputum processing method for detection of tuberculosis in developing countries. METHODS: A sample processing method was developed in which acid fast bacilli were killed with 1% sodium hypochlorite and concentrated by flotation on a layer of xylene before staining by the Ziehl Neelsen or auramine O methods. RESULTS: Best results were obtained by auramine O staining after flotation. Staining by the Ziehl Neelsen method after flotation gave better results than direct Ziehl Neelsen staining without flotation. CONCLUSIONS: The flotation method with Ziehl Neelsen staining offers advantages for smear preparation in the tuberculosis control programmes of developing countries.
Authors: M A Fischl; R B Uttamchandani; G L Daikos; R B Poblete; J N Moreno; R R Reyes; A M Boota; L M Thompson; T J Cleary; S Lai Journal: Ann Intern Med Date: 1992-08-01 Impact factor: 25.391
Authors: M L Pearson; J A Jereb; T R Frieden; J T Crawford; B J Davis; S W Dooley; W R Jarvis Journal: Ann Intern Med Date: 1992-08-01 Impact factor: 25.391
Authors: Padmapriya P Banada; Satheesh K Sivasubramani; Robert Blakemore; Catharina Boehme; Mark D Perkins; Kevin Fennelly; David Alland Journal: J Clin Microbiol Date: 2010-08-18 Impact factor: 5.948